Microbial production of xylanase is gaining the commercial importance, due to its wide range of applications from paper and pulp to food and feed industries. Streptomyces geysiriensis was used for the production of extracellular xylanase from lignocellulosic substrates such as rice bran and saw dust, under solid-state fermentation. The influence of pH, temperature and incubation period for the maximum production of xylanase was investigated with 1:2 (w/v) of substrate to moisture ratio at 100 rpm shaking conditions. The maximum production was recorded after 5 days of fermentation with pH 8.0 at 40 °C. The scale-up was done based on the results of optimized parameters using 3 L Applikon autoclavable bioreactor with maximum yield of 186 U/ml after 4 days of fermentation. Extracellular xylanase was separated by partitioning in aqueous two-phase system consisting of 20% polyethylene glycol 6000 and 12% K₂HPO₄ with maximum yield of 93.97%. The investigation of the effect of pH and temperature and its incubation time showed that xylanase was retained its activity in a pH range of 6.5–8.5, with thermal stability from 20 °C to 60 °C up to 180 min. The presence of metal ions was found to inhibit the activity of xylanase especially Cu²⁺ and Zn²⁺. Xylanase was stable both at 4 °C and room temperature (35 °C) for 30 and 9 days respectively. The kinetic parameters K_m (0.48 mg/ml) and V_max (8.33 U/mg) were determined using birchwood xylan as substrate.

木聚糖酶的微生物生产正获得商业重要性，这是因为其在纸和纸浆到食品和饲料工业的广泛应用。吉里链霉菌在固态发酵条件下，“麦芽糖”用于从木质纤维素底物（如米糠和锯末）生产胞外木聚糖酶。在100 rpm摇动条件下，以1：2（w / v）的底物与水分的比例研究了pH，温度和孵育时间对木聚糖酶最大产量的影响。在40°C下于pH 8.0发酵5天后，记录了最大产量。在发酵4天后，使用3 L Applikon可高压灭菌的生物反应器，根据优化参数的结果进行放大，最大产量为186 U / ml。通过在由20％聚乙二醇6000和12％K ₂ HPO ₄组成的含水两相系统中分配来分离细胞外木聚糖酶最大产率为93.97％。对pH和温度及其孵育时间的影响进行的研究表明，木聚糖酶在6.5–8.5的pH范围内保持其活性，在20°C至60°C的温度下长达180分钟具有热稳定性。发现金属离子的存在抑制木聚糖酶特别是Cu ²⁺和Zn ²⁺的活性。木聚糖酶分别在4°C和室温（35°C）下稳定30天和9天。以桦木木聚糖为底物测定动力学参数K _m（0.48 mg / ml）和V _max（8.33 U / mg）。