Feline anaplasmosis is considered as an emerging tick-borne disease of zoonotic potential. The aim of current study was to investigate the molecular prevalence of anaplasmosis, associated risk factors, and alterations in hematological parameters of domestic cats from Lahore, Pakistan. Blood samples of 100 domestic cats from district Lahore were examined microscopically and the extracted genomic DNA from each sample was processed for the amplification of 16 S rRNA gene of Anaplasma. PCR confirmed isolates were purified for sequencing. The data regarding the risk factors was collected in a predesigned questionnaire and statistically analyzed by logistic regression analysis. The study found a molecular prevalence of 13% (13/100) among analyzed blood samples. The nucleotide analysis of Anaplasmataceae species sequences amplified by PCR showed high resemblance (99%) with isolates from Korea, Japan, Malaysia, Philippines, and India. The potential risk factors found to be significantly associated (p < 0.05) with disease dynamics based on the Chi-Square test were tick infestation on studied animals, previous tick history, tick control status, house hygiene, and housing type. A significant (p < 0.05) decrease in the number of platelets, erythrocytes, hemoglobin level, and pack cell volume was observed in cats suffering from anaplasmosis compared to the healthy ones. The current study is the first report of Anaplasma infection in domestic cats of Pakistan. This study will be effectual in designing the control strategies for this disease.

猫厌氧菌病被认为是一种新兴的由tick传播的人畜共患病。当前研究的目的是调查巴基斯坦拉合尔家猫的厌氧菌分子流行率，相关的危险因素以及血液学参数的变化。显微镜检查了100名来自拉合尔地区的家猫的血液样本，并对每个样本中提取的基因组DNA进行了处理，以扩增Anaplasma的16 S rRNA基因。。PCR证实的分离物被纯化用于测序。有关风险因素的数据收集在预先设计的调查表中，并通过逻辑回归分析进行统计分析。该研究发现，在分析的血液样本中，分子患病率为13％（13/100）。通过PCR扩增的无形科物种序列的核苷酸分析显示，与来自韩国，日本，马来西亚，菲律宾和印度的分离株高度相似（99％）。 根据卡方检验，发现与疾病动态显着相关的潜在危险因素（p <0.05）是对研究动物的tick虫侵扰，既往tick史，tick虫控制状况，房屋卫生和房屋类型。显着性（p <0.05）与健康猫相比，患有厌氧菌病的猫的血小板，红细胞，血红蛋白水平和包装细胞体积减少。当前的研究是巴基斯坦家猫中无形体感染的首次报道。这项研究将有效地设计该疾病的控制策略。