Leptospirosis is a zoonotic disease with worldwide distribution. The disease affects dairy and beef cattle, causing infertility, abortion, and reduced milk yield. A cross-sectional study was conducted to determine the seroprevalence of leptospirosis in cattle and the associated risk factors in the province of Manabí, Ecuador. Serum samples from 749 animals from 55 cattle herds were analyzed using the microscopic agglutination test (MAT). Animals were considered positive when titers were ≥ 1:100. The association between the potential risk factors and the positive Leptospira result was modeled at both animal and herd level using a generalized linear model with a binomial distribution and logarithmic link. The seroprevalence was 56.21% at the individual level and 98.18% at the herd level. The most prevalent serovars were Pomona (28.57%) and Icterohaemorragiae (22.30%). At the animal level, only the age was associated with leptospirosis seropositivity. Seroprevalence in animals over three years of age was 1.197 (95% confidence intervals (CI), 1.032 – 1.390), higher compared to animals up to three years old. The seroprevalence of Leptospira spp. was higher in farms with no veterinary assistance (PR = 1.209; 95%CI 1.053 – 1.388) and without a vaccination program against Leptospira (PR = 1.399; 95%CI 1.09 - 1.794). In addition, herds from Junín canton had a significantly higher seroprevalence of Leptospira spp (PR = 1.548; 95%CI 1.213 - 1.977) compared to the Bolívar canton, which had the lowest seroprevalence. In conclusion, more than half of the animals were positive to Leptospiraspp, and almost all herds had at least one positive animal. Furthermore, veterinary assistance and vaccination of cattle must be considered as essential aspects of the disease control program.

钩端螺旋体病是一种人畜共患病，分布在世界各地。该病影响奶牛和肉牛，导致不孕，流产和牛奶产量下降。进行了一项横断面研究，以确定厄瓜多尔马纳比省牛的钩端螺旋体病的血清阳性率及其相关危险因素。使用显微镜凝集试验（MAT）对来自55个牛群的749只动物的血清样本进行了分析。当滴度≥1：100时，动物被认为是阳性的。使用具有二项分布和对数关系的广义线性模型，在动物和畜群水平上对潜在危险因素与钩端螺旋体阳性结果之间的关联进行建模。个体的血清阳性率为56.21％，而从畜群的血清阳性率为98.18％。最普遍的血清型是Pomona（28。57％）和Icterohaemorragiae（22.30％）。在动物方面，只有年龄与钩端螺旋体血清阳性有关。3岁以上动物的血清阳性率是1.197（95％置信区间（CI），1.032-1.390），高于3岁以下动物。的血清阳性率钩端螺旋体属。在没有兽医辅助的农场（PR = 1.209； 95％CI 1.053 – 1.388）且没有针对钩端螺旋体的疫苗接种计划的农场（PR = 1.399； 95％CI 1.09-1.794）的水平更高。此外，与血清感染率最低的玻利瓦尔州相比，来自胡宁州的牛群的钩端螺旋体血清阳性率更高（PR = 1.548； 95％CI 1.213-1.977）。总之，超过一半的动物对钩端螺旋体属呈阳性，几乎所有畜群都至少有一只阳性动物。此外，兽医援助和牛疫苗接种必须视为疾病控制计划的重要方面。