In plants, both hyperosmolality and salt stress induce cytosolic calcium increases within seconds, referred to as the hyperosmolality-induced [Ca²⁺]_cyt increases, OICI_cyt, and salt stress-induced [Ca²⁺]_cyt increases, SICI_cyt. Previous studies have shown that Arabidopsis reduced hyperosmolality-induced [Ca²⁺]_i increase 1 (OSCA1.1) encodes a hyperosmolality-gated calcium-permeable channel that mediates OICI_cyt in guard cells and root cells. Multiple OSCA members exist in plants; for example, Oryza sativa has 11 OsOSCAs genes, indicating that OSCAs have diverse biological functions. Here, except for OsOSCA4.1, ten full-length OsOSCAs were separately subcloned, in which OsOSCA1.4 was exclusively localised to the plasma membrane and other nine OsOSCAs-eYFP co-localised with an endoplasmic reticulum marker in Arabidopsis mesophyll protoplasts. OsOSCA1.4 was further identified as a calcium-permeable ion channel that activates an inward current after receiving an osmotic signal exerted by hyperosmolality or salt stress, and mediates OICI_cyt and SICI_cyt in human embryonic kidney 293 (HEK293) cells. Moreover, overexpression of OsOSCA1.4 in Arabidopsis osca1 mutant complemented osmotic Ca²⁺ signalling, root growth, and stomatal movement in response to hyperosmolality and salt stress. These results will facilitate further study of OsOSCA-mediated calcium signalling and its distinct roles in rice growth and development.

在植物中，高渗透压和盐胁迫都会在几秒钟内诱导细胞溶质钙增加，称为高渗透压诱导的 [Ca ²⁺ ]_细胞色素增加，OICI 细胞_色素和盐胁迫诱导的 [Ca ²⁺ ]_细胞色素增加，SICI_细胞色素。先前的研究表明，拟南芥降低高渗透压诱导的 [Ca ²⁺ ] _i增加 1 ( OSCA1.1 ) 编码高渗透压门控钙渗透通道，该通道介导保卫细胞和根细胞中的OICI细胞_色素。工厂中存在多个OSCA成员；例如，水稻有 11 个OsOSCAs基因，表明 OSCAs 具有多种生物学功能。在这里，除了 OsOSCA4.1 外，十个全长OsOSCAs被单独亚克隆，其中 OsOSCA1.4 仅定位于质膜，其他九个 OsOSCAs-eYFP 与拟南芥叶肉原生质体中的内质网标记共定位。OsOSCA1.4 被进一步鉴定为钙可渗透离子通道，在接收高渗透压或盐胁迫施加的渗透信号后激活内向电流，并介导人胚胎肾 293 (HEK293) 细胞中的OICI 细胞_色素和 SICI_细胞色素。此外，OsOSCA1.4在拟南芥 osca1 中的过表达突变体补充渗透性 Ca ²⁺信号、根生长和气孔运动以响应高渗透压和盐胁迫。这些结果将有助于进一步研究 OsOSCA 介导的钙信号及其在水稻生长发育中的独特作用。