Somatic embryogenesis of Fraxinus mandshurica has the problems of low somatic embryo (SE) yield, unsynchronized SE development, and a high percentage of deformed SEs. We aimed to improve F. mandshurica SE production by synchronizing SE development, improving SE quality, and inducing root formation to obtain complete regenerated plants. Cotyledons of immature zygotic embryos of F. mandshurica were induced to form callus and then SEs. The SE induction percentage from explants differed among 32 mother trees, and the one with the highest SE induction percentage (29.8%) was used for further experiments. The highest callus induction percentage was 94.2% on ½-strength Murashige and Skoog medium (MS½) supplemented with 0.15 mg·L⁻¹ naphthalene acetic acid. The highest callus proliferation coefficient (240.5) was obtained on McCown’s Woody Plant Medium containing 0.1 mg·L⁻¹ 6-benzyl adenine and 0.15 mg·L⁻¹ 2, 4-dichlorophenoxyacetic acid. The highest number of SEs (1020.5 g⁻¹ fresh weight) was obtained on MS½ medium supplemented with 1 mg·L⁻¹ 6-benzyladenine. The highest number of cotyledon embryos (397/g fresh weight) was obtained by incubating materials on medium containing 1 mg·L⁻¹ abscisic acid and then applying a drying treatment. The cotyledon embryos were milky white, uniformly sized (average length 4.7 mm), and 80% of them were normal. The SE rooting percentage on ½MS medium containing 0.01 mg·L⁻¹ NAA was 37.5%. Overall, the germination percentage of SEs was 26.4%, and complete regenerated plants were obtained after transplanting and acclimation. These results provide more possibilities for the preservation and breeding of F. mandshurica.

小号的omatic胚胎发生水曲柳具有低的体细胞胚（SE）产率的问题，不同步的SE发展，和变形的SE的高百分比。我们的目标是通过同步SE发展，提高SE质量和诱导根形成以获得完整的再生植株来改善水曲柳SE的生产。的未成熟合子胚的子叶水曲柳被诱导以形成愈伤组织，然后的SE。外植体中的SE诱导百分比在32棵母树之间有所不同，并且将具有最高SE诱导百分比（29.8％）的一棵用于进一步的实验。在补充0.15 mg·L ^-1的1/2强度Murashige和Skoog培养基（MS½）上，最高的愈伤组织诱导率为94.2％。萘乙酸。在含有0.1mg·L ^-1的6-苄基腺嘌呤和0.15mg·L ^-1的2，4-二氯苯氧基乙酸的McCown的Woody植物培养基上获得最高的愈伤组织增殖系数（240.5）。在补充有1 mg·L ^-1的6-苄基腺嘌呤的MS½培养基上获得最高数量的SEs（1020.5 g ^-1鲜重）。通过在含有1 mg·L ^-1脱落酸的培养基上孵育材料，然后进行干燥处理，可以得到最高数量的子叶胚（397 / g鲜重）。子叶胚为乳白色，大小均一（平均长度4.7 mm），其中80％正常。在含有0.01 mg·L ^{-1的1 /} 2MS培养基上的SE生根百分比NAA为37.5％。总体而言，SEs的发芽率为26.4％，在移植和驯化后获得了完整的再生植物。这些结果提供的保护和繁育更多的可能性水曲柳。