Immunohistochemical staining of tissue sections is a vital technique in pathological diagnostics and theranostics. Several kinds of detection systems are available—each of them with their advantages and disadvantages. Here we present the results of a study assessing a prototype immunohistochemical detection technology (PIDT) for visualization of antigens in tissue sections. Different tumor tissues (n = 11) were stained with selected antibodies (n = 30) and a subset of these under different fixation conditions. The staining properties were assessed according to six staining quality parameters (signal distribution, intensity, tissue and slide background, acutance, clarity of details, and subcellular morphological details), and the results were compared with those of a well-established detection system (EnVision FLEX). Overall, both detection methods revealed good to optimal results regarding the evaluated parameters even under unfavorable fixation conditions. However, with the prototype detection technology a quicker turnaround time was reached primarily due to shorter primary antibody incubation times. Moreover, PIDT-stained tissues showed higher signal intensity and a uniform signal distribution over the tissue slide, still, with well-preserved tissue morphology and without impairing the gradation of staining intensity of different cell types. In particular, the prototype detection technology performed better in poorly or delayed fixed tissue. In situations where rapid and profound results are in demand, and particularly in the context of a small laboratory setting, this prototype detection technology could be a useful addition to the established detection systems.

组织切片的免疫组织化学染色是病理诊断和诊断学中的一项重要技术。可以使用多种检测系统，每种检测系统都有其优点和缺点。在这里，我们介绍评估原型免疫组织化学检测技术（PIDT）的可视化组织切片中抗原的研究结果。不同肿瘤组织（n  = 11）用所选抗体染色（n = 30），以及在不同注视条件下的其中一部分。根据六个染色质量参数（信号分布，强度，组织和玻片背景，尖锐度，细节的清晰度和亚细胞形态学细节）评估了染色特性，并将其结果与完善的检测系统（EnVision）进行了比较柔性）。总体而言，即使在不利的固定条件下，两种检测方法也能显示出与评估参数有关的良好至最佳结果。但是，使用原型检测技术，主要是由于较短的一抗孵育时间，因此可以缩短周转​​时间。而且，PIDT染色的组织在组织载玻片上显示出更高的信号强度和均匀的信号分布。具有良好保存的组织形态，并且不会损害不同细胞类型的染色强度的等级。特别是，原型检测技术在不良或延迟的固定组织中表现更好。在需要快速而深刻的结果的情况下，尤其是在实验室规模较小的情况下，这种原型检测技术可能是已建立的检测系统的有用补充。