Translationally controlled tumor protein (TCTP) or fortilin is a highly conserved protein among eukaryotes and has important biological roles including anti-apoptotic activity which involves the elimination of virus-infected cells. In this study, the full-length cDNA of Macrobrachium rosenbergii TCTP (MrTCTP) was isolated. The cDNA sequence consisted of 783 bp with a 507-bp open reading frame encoding 168 amino acids. MrTCTP demonstrated all major characteristics of TCTP which include (1) highly homologous to TCTP protein family, (2) carrying two TCTP signatures, and (3) contained highly conserved amino acid sequences. The predicted structure of MrTCTP also revealed typical structure of TCTP which had three major domains including ß-stranded core domain, helical domain, and flexible loop. The predicted molecular mass of MrTCTP was 18.94 kDa and sequence displays 83.93 and 78.57% identity to that of Eriocheir sinensis TCTP and Fenneropenaeus chinensis TCTP, respectively. Phylogenetic analysis revealed that the deduced amino acid sequence of MrTCTP was clustered into the invertebrate branch along with the sequences from arthropods and crustaceans. The mRNA expression showed that MrTCTP expressed in every tissue examined, preferentially in hepatopancreas and muscle. In addition, MrTCTP expression in the muscle was upregulated at 1 to 4 days after infection with M. rosenbergii nodavirus (MrNV). Finally, silencing of MrTCTP increased mortality rates post-MrNV challenge. Taken together, the study shows that MrTCTP may play an important role in shrimp immune response against MrNV infection.

翻译控制的肿瘤蛋白（TCTP）或Fortilin是真核生物中高度保守的蛋白，具有重要的生物学作用，包括抗凋亡活性，涉及消除感染病毒的细胞。在这项研究中，罗氏沼虾TCTP（TCTP先生）的全长cDNA被分离。cDNA序列由783 bp和507 bp的开放阅读框组成，编码168个氨基酸。TCTP先生展示了TCTP的所有主要特征，包括（1）与TCTP蛋白家族高度同源，（2）带有两个TCTP签名，以及（3）包含高度保守的氨基酸序列。先生的预测结构TCTP还揭示了TCTP的典型结构，它具有三个主要结构域，包括ß链核心结构域，螺旋结构域和柔性环。TCTP先生的预测分子量为18.94 kDa，其序列分别与中华绒螯蟹TCTP和中华粉刺TCTP分别具有83.93和78.57％的同一性。系统发育分析表明，TCTP先生的推导氨基酸序列与节肢动物和甲壳类动物的序列一起聚集成无脊椎动物分支。mRNA表达表明MrTCTP在每个检查的组织中表达，优先在肝胰腺和肌肉中表达。此外，MrTCTP在感染罗氏沼虾诺达病毒（Mr NV）后的1至4天，肌肉中的表达上调。最后，对TCTP先生采取沉默措施会增加NV先生挑战后的死亡率。两者合计，研究表明TCTP先生可能在虾对NV先生的免疫反应中起重要作用。