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Evaluation of an Ag85B Immunosensor with Potential for Electrochemical Mycobacterium Tuberculosis Diagnostics
ECS Journal of Solid State Science and Technology ( IF 2.2 ) Pub Date : 2020-08-05 , DOI: 10.1149/2162-8777/aba993
Brian Murphy 1 , Eithne Dempsey 2
Affiliation  

Tuberculosis remains a major global health concern, especially in the developing world, and monitoring/early detection of the disease relies on low cost technologies that provide rapid and accurate results. Mycobacterium tuberculosis is the responsible bacterial pathogen and it is currently estimated by the World Health Organisation (WHO), that one quarter of the world's population, mainly in the developing world, is infected with TB. The overall aim of this work was to advance a screening electrochemical sensor for label free detection of Ag85B, a member of the Antigen 85 complex—major secretary protein of M. tuberculosis and biomarker for disease. An indirect ELISA Ag85B assay was optimised with capture antibody and antigen levels determined via a checkerboard titration (0.625 μ g ml −1 and 2.5 μ g ml −1 respectively). Following assay development, crosslinking of the bioreceptor Anti-Ag85B onto electrochemically deposited gold nano...
更新日期:2020-08-06
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