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HDA6-dependent histone deacetylation regulates mRNA polyadenylation in Arabidopsis.
Genome Research ( IF 7 ) Pub Date : 2020-10-01 , DOI: 10.1101/gr.255232.119
Juncheng Lin , Fu-Yu Hung , Congting Ye , Liwei Hong , Yuan-Hsin Shih , Keqiang Wu , Qingshun Q. Li

Eukaryotic histone deacetylation, critical for maintaining nucleosome structure and regulating gene expression, is mediated by histone deacetylases (HDACs). Although nucleosomes have been reported to regulate mRNA polyadenylation in humans, the role of HDACs in regulating polyadenylation has not been uncovered. Taking advantage of phenotypic studies on Arabidopsis, HDA6 (one of HDACs) was found to be a critical part of many biological processes. Here, we report that HDA6 affects mRNA polyadenylation in Arabidopsis. Poly(A) sites of up-regulated transcripts are closer to the histone acetylation peaks in hda6 compared to the wild-type Col-0. HDA6 is required for the deacetylation of histones around DNA on nucleosomes, which solely coincides with up-regulated or uniquely presented poly(A) sites in hda6. Furthermore, defective HDA6 results in an overrepresentation of the canonical poly(A) signal (AAUAAA) usage. Chromatin loci for generating AAUAAA-type transcripts have a comparatively low H3K9K14ac around poly(A) sites when compared to other noncanonical poly(A) signal–containing transcripts. These results indicate that HDA6 regulates polyadenylation in a histone deacetylation–dependent manner in Arabidopsis.

中文翻译:

HDA6 依赖性组蛋白去乙酰化调节拟南芥中的 mRNA 多聚腺苷酸化。

真核组蛋白去乙酰化对维持核小体结构和调节基因表达至关重要,由组蛋白去乙酰化酶 (HDAC) 介导。尽管据报道核小体可调节人类的 mRNA 多聚腺苷酸化,但尚未发现 HDAC 在调节多聚腺苷酸化中的作用。利用对拟南芥的表型研究,发现 HDA6(HDAC 之一)是许多生物过程的关键部分。在这里,我们报告 HDA6 影响拟南芥中的mRNA 多聚腺苷酸化。上调转录本的 Poly(A) 位点更接近hda6 中的组蛋白乙酰化峰与野生型 Col-0 相比。HDA6 是核小体 DNA 周围组蛋白脱乙酰化所必需的,这仅与hda6 中上调或独特呈现的 poly(A) 位点相吻合。此外,有缺陷的 HDA6 会导致规范 poly(A) 信号 (AAUAAA) 使用率过高。与其他包含非典型 poly(A) 信号的转录本相比,用于生成 AAUAAA 型转录本的染色质基因座在 poly(A) 位点周围具有相对较低的 H3K9K14ac。这些结果表明 HDA6 在拟南芥中以组蛋白去乙酰化依赖的方式调节多聚腺苷酸化。
更新日期:2020-10-02
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