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Noninvasive Multielectrode Array for Brain and Spinal Cord Local Field Potential Recordings from Live Zebrafish Larvae.
Zebrafish ( IF 2 ) Pub Date : 2020-08-13 , DOI: 10.1089/zeb.2020.1874 Danielle L Tomasello 1 , Hazel Sive 1, 2
Zebrafish ( IF 2 ) Pub Date : 2020-08-13 , DOI: 10.1089/zeb.2020.1874 Danielle L Tomasello 1 , Hazel Sive 1, 2
Affiliation
Zebrafish are an important and expanding experimental system for brain research. We describe a noninvasive electrophysiology technique that can be used in living larvae to measure spontaneous activity in the brain and spinal cord simultaneously. This easy-to-use method uses a commercially available multielectrode array to detect local field potential parameters, and allows for relative coordinated (network) measurements of activity. We demonstrate sensitivity of this system by measuring activity in larvae treated with the antiepileptic drug valproic acid. Valproic acid decreased larval movement and startle response, and decreased spontaneous brain activity. Spinal cord activity did not change after treatment, suggesting valproic acid primarily affects brain function. The observed differences in brain activity, but not spinal cord activity, after valproic acid treatment indicates that brain activity differences are not a secondary effect of decreased startle response and movement. We provide a step-by-step protocol for experiments presented that a novice could easily follow. This electrophysiological method will be useful to the zebrafish neuroscience community.
中文翻译:
用于活斑马鱼幼虫大脑和脊髓局部场电位记录的无创多电极阵列。
斑马鱼是大脑研究中重要且不断扩展的实验系统。我们描述了一种无创电生理学技术,可用于活体幼虫,同时测量大脑和脊髓的自发活动。这种易于使用的方法使用市售的多电极阵列来检测局部场电位参数,并允许相对协调(网络)活动测量。我们通过测量用抗癫痫药物丙戊酸治疗的幼虫的活性来证明该系统的敏感性。丙戊酸减少了幼虫的运动和惊吓反应,并减少了自发的大脑活动。治疗后脊髓活动没有变化,表明丙戊酸主要影响大脑功能。丙戊酸治疗后观察到的大脑活动差异,但脊髓活动没有差异,表明大脑活动差异并不是惊吓反应和运动减少的继发效应。我们提供了一个新手可以轻松遵循的分步实验方案。这种电生理学方法将对斑马鱼神经科学界有用。
更新日期:2020-08-17
中文翻译:
用于活斑马鱼幼虫大脑和脊髓局部场电位记录的无创多电极阵列。
斑马鱼是大脑研究中重要且不断扩展的实验系统。我们描述了一种无创电生理学技术,可用于活体幼虫,同时测量大脑和脊髓的自发活动。这种易于使用的方法使用市售的多电极阵列来检测局部场电位参数,并允许相对协调(网络)活动测量。我们通过测量用抗癫痫药物丙戊酸治疗的幼虫的活性来证明该系统的敏感性。丙戊酸减少了幼虫的运动和惊吓反应,并减少了自发的大脑活动。治疗后脊髓活动没有变化,表明丙戊酸主要影响大脑功能。丙戊酸治疗后观察到的大脑活动差异,但脊髓活动没有差异,表明大脑活动差异并不是惊吓反应和运动减少的继发效应。我们提供了一个新手可以轻松遵循的分步实验方案。这种电生理学方法将对斑马鱼神经科学界有用。