Oral bacterial infection represents the leading cause of the gradual destruction of tooth and periodontal structures anchoring the teeth. Lately, injectable hydrogels have gained increased attention as a promising minimally invasive platform for localized delivery of personalized therapeutics. Here, an injectable and photocrosslinkable gelatin methacryloyl (GelMA) hydrogel is successfully engineered with ciprofloxacin (CIP)-eluting short nanofibers for oral infection ablation. For this purpose, CIP or its β-cyclodextrin (β-CD)-inclusion complex (CIP/β-CD-IC) has been incorporated into polymeric electrospun fibers, which were subsequently cut into short nanofibers, and then embedded in GelMA to obtain an injectable hybrid antimicrobial hydrogel. Thanks to the solubility enhancement of CIP by β-CD-IC and the tunable degradation profile of GelMA, the hydrogels promote localized, sustained, and yet effective cell-friendly antibiotic doses, as measured by a series of bacterial assays that demonstrated efficacy in attenuating the growth of Gram-positive Enterococcus faecalis. Altogether, we foresee significant potential in translating this innovative hybrid hydrogel as an injectable platform technology that may have broad applications in oral infection ablation, such as periodontal disease and pulpal pathology.

中文翻译：

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混合抗菌水凝胶作为口腔感染消融的注射疗法。

口腔细菌感染是导致牙齿和锚固牙齿的牙周结构逐渐破坏的主要原因。最近，可注射的水凝胶作为一种有针对性的微创平台，可用于个性化治疗的局部递送，引起了越来越多的关注。在这里，可注射和可光交联的明胶甲基丙烯酰基（GelMA）水凝胶已成功地与环丙沙星（CIP）洗脱的短纳米纤维一起工程化，可用于口腔感染消融。为此，已将CIP或其β-环糊精（β-CD）包合物（CIP /β-CD-IC）掺入聚合物电纺纤维中，然后将其切成短的纳米纤维，然后包埋在GelMA中以获得可注射的混合抗菌水凝胶。由于通过β-CD-IC增强了CIP的溶解度以及GelMA的可调节降解特性，粪肠球菌。总的来说，我们预见到这种创新的混合水凝胶作为可注射平台技术的巨大潜力，该技术可广泛应用于口腔感染消融，例如牙周疾病和牙髓病理。