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Reverse expression pattern of sirtuin-1 and histone deacetylase-9 in coronary artery disease
Archives of Physiology and Biochemistry ( IF 3 ) Pub Date : 2020-08-06
Laleh Heidari, Sayyed Mohammad Hossein Ghaderian, Milad Bastami, Shadi Hosseini, Saeed Alipour Parsa, Sahel Heidari, Hossein Jafari, Nasim Sohrabifar, Maryam Pirhoushiaran

Background

SIRT1 and HDAC 9 genes are related to inflammation and may contribute to the pathogenesis of coronary artery disease (CAD). We aimed to evaluate the expression level, methylation profile and polymorphisms of these genes in CAD patients.

Methods

In this study, 50 CAD patients and 50 healthy individuals were recruited. The expression level change was evaluated using the TaqMan Real-Time PCR method. The methylation of genes promoter and genotyping of polymorphisms were evaluated by the HRM.

Results

The expression level of SIRT1 was reduced while the HDAC9 expression level showed a significant elevation (p < .001). The SIRT1 gene promoter was hypomethylated and the HDAC9 gene promoter was hypermethylated in CAD patients. Also, CG + GG genotype in SIRT1 and both genotypes in the HDAC9 gene were associated with expression change.

Conclusions

SIRT1 and HDAC9 genes, expression changes can be suggested as a potential biomarker for CAD detection.



中文翻译:

sirtuin-1和组蛋白脱乙酰基酶9在冠心病中的反向表达模式

背景

SIRT1HDAC 9基因与炎症有关,可能与冠状动脉疾病(CAD)的发病机理有关。我们旨在评估这些基因在CAD患者中的表达水平,甲基化谱和多态性。

方法

在这项研究中,招募了50名CAD患者和50名健康个体。使用TaqMan实时PCR方法评估表达水平的变化。通过HRM评估基因启动子的甲基化和多态性的基因型。

结果

SIRT1的表达水平降低,而HDAC9的表达水平显着升高(p  <.001)。在CAD患者中,SIRT1基因启动子被低甲基化,而HDAC9基因启动子被高甲基化。另外,SIRT1中的CG + GG基因型和HDAC9基因的两种基因型均与表达变化相关。

结论

SIRT1HDAC9基因的表达变化可能被建议作为CAD检测的潜在生物标记。

更新日期:2020-08-06
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