Abstract

Pretreatment can improve the hydrolysis efficiency of cellulose, in which biological pretreatment plays an important role. In the present study, we uncovered that Rhodococcus has the ability of lignin degradation, which can decompose lignin and serve as a carbon source to meet the needs of its own growth. We used Rhodococcus to pretreat the corn stalks and evaluate the effect on cellulose hydrolysis. The concentration of reducing sugar produced by the hydrolysis of corn stalk after pretreatment of Rhodococcus is 2.95 g/L. SEM imaging showed that Rhodococcus pretreatment resulted the surface of corn stalk to be no longer complete, some lamellar structures fall off, and leave obvious traces, and obvious delamination was found at the edge of the fault. AFM imaging showed that the pretreatment changed the lignin structure of the corn stalk material surface, resulting in a higher surface roughness of 9.37. These results indicated that Rhodococcus pretreatment can improve the saccharification efficiency of cellulose by removing lignin and increasing the surface roughness of the material.

抽象的

预处理可以提高纤维素的水解效率，其中生物预处理起着重要的作用。在本研究中，我们发现红球菌具有木质素降解的能力，可以分解木质素并作为满足自身生长需求的碳源。我们使用红球菌对玉米秸秆进行预处理，并评估其对纤维素水解的影响。红球菌预处理后玉米秸秆水解产生的还原糖浓度为2.95 g / L。SEM图像显示红球菌预处理导致玉米秸秆表面不再完整，一些层状结构脱落，并留下明显痕迹，并且在断层边缘发现了明显的分层。原子力显微镜成像表明，预处理改变了玉米秸秆材料表面的木质素结构，导致表面粗糙度更高，为9.37。这些结果表明，红球菌预处理可以通过去除木质素和增加材料的表面粗糙度来提高纤维素的糖化效率。