Abstract

Here, we studied the protective effect of rutin (RUT) against testicular damage caused by busulfan (BUS) in rats. Adult male Wistar rats were intraperitoneally injected with BUS (4 mg/kg body weight at day 7 and 14), and then treated with RUT (30 mg/kg body weight) by gavage thrice weekly for 60 days. The results showed that BUS-induced increase in 3β-hydroxysteroid dehydrogenase (3β-HSD) was significantly decreased by RUT, whereas 17β-HSD activity and plasma testosterone concentration remained unaffected (p > 0.05). It was also observed that RUT inhibited BUS-induced increase in nitrite concentrations and myeloperoxidase enzyme activities in the plasma and testes (p < 0.05). Similarly, BUS-induced decrease in glutathione and increase in malondialdehyde concentrations in the testes were significantly normalized to control values by RUT. Finally, RUT administration showed some tendency to improve the architecture of the seminiferous epithelium of the rat’s testes after BUS treatment. Overall, RUT inhibited BUS-induced oxidative damage and inflammation in the testis of an experimental rat model.

摘要

在这里，我们研究了芦丁 (RUT) 对白消安 (BUS) 对大鼠睾丸损伤的保护作用。成年雄性 Wistar 大鼠腹膜内注射 BUS（第 7 天和第 14 天时为 4 mg/kg 体重），然后每周 3 次通过管饲法用 RUT（30 mg/kg 体重）处理 60 天。结果显示，BUS诱导的3β-羟基类固醇脱氢酶（3β-HSD）的增加被RUT显着降低，而17β-HSD活性和血浆睾酮浓度不受影响（p  >0.05）。还观察到 RUT 抑制 BUS 诱导的血浆和睾丸中亚硝酸盐浓度和髓过氧化物酶活性的增加（p < 0.05)。同样，BUS 诱导的睾丸中谷胱甘肽的降低和丙二醛浓度的增加通过 RUT 显着标准化为对照值。最后，在 BUS 治疗后，RUT 给药显示出改善大鼠睾丸生精上皮结构的一些趋势。总体而言，RUT 在实验大鼠模型的睾丸中抑制了 BUS 诱导的氧化损伤和炎症。