This study aimed to assess the effect of petrol and the Fyre Zyme reagent on selected chemical and biochemical properties of loamy sand. The experiment was conducted under laboratory conditions. First, petrol was introduced into the soil at doses of 0 and 50 g k^-1dry matter (DM). Next, 6% Fyre-Zyme enzyme reagent solution was added to the samples contaminated and uncontaminated with petrol, in the following combinations: 0 (control), once at 40 cm³ kg^–1 DM, twice at 20 cm³ kg^-1 DM at two-week intervals, and four times at 10 cm³ kg^-1 DM at weekly intervals. Contamination of loamy sand with petrol caused slight changes in the determined chemical parameters and stimulated dehydrogenase activities, but inhibited the activity of phosphatases. The introduction of the enzyme reagent into the soil increased the C_org and N_tot content. The greatest changes were observed in the activity of phosphatases. The receiver operating characteristic (ROC) curves revealed that the application of the enzyme reagent at the application of 4 × 10 cm³ kg^-1 DM was the most beneficial. However, the results of the η² analysis indicate that the greatest influence on the determined experimental parameters was found in the soil contaminated with petrol.

中文翻译：

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酶试剂对石油污染土壤生物刺激后的理化参数分析

这项研究旨在评估汽油和Fyre Zyme试剂对壤土沙的选定化学和生化特性的影响。实验是在实验室条件下进行的。首先，将汽油以0和50 gk ^-1干物质（DM）的剂量引入土壤。接下来，将6％Fyre-Zyme酶试剂溶液添加到受汽油污染和未污染的样品中，组合如下：0（对照），一次在40 cm ³ kg ^–1 DM，两次在20 cm ³ kg ^-1 DM每两周一次，在10 cm ³ kg ^-1时四次每周间隔DM。汽油对壤土沙的污染导致所确定的化学参数略有变化，并刺激了脱氢酶的活性，但抑制了磷酸酶的活性。将酶试剂引入土壤增加了C _org和N _tot含量。磷酸酶的活性变化最大。接收器工作特性（ROC）曲线表明，在4×10 cm ³ kg ^-1 DM的应用中酶试剂的应用是最有益的。然而，η结果²分析表明，所确定的实验参数影响最大的污染与汽油的土壤被发现。