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Reproductive Outcomes and Endocrine Profile in Artificially Inseminated versus Embryo Transferred Cows.
Animals ( IF 3 ) Pub Date : 2020-08-06 , DOI: 10.3390/ani10081359
Jordana S Lopes 1, 2 , Estefanía Alcázar-Triviño 3 , Cristina Soriano-Úbeda 1 , Meriem Hamdi 4 , Sebastian Cánovas 1, 2 , Dimitrios Rizos 4 , Pilar Coy 1, 2
Affiliation  

The increasing use of in vitro embryo production (IVP) followed by embryo transfer (ET), alongside with cryopreservation of embryos, has risen concerns regarding the possible altered pregnancy rates, calving or even neonatal mortality. One of the hypotheses for these alterations is the current culture conditions of the IVP. In an attempt to better mimic the physiological milieu, embryos were produced with female reproductive fluids (RF) as supplements to culture medium, and another group of embryos were supplemented with bovine serum albumin (BSA) as in vitro control. Embryos were cryopreserved and transferred while, in parallel, an in vivo control (artificial insemination, AI) with the same bull used for IVP was included. An overview on pregnancy rates, recipients’ hormonal levels, parturition, and resulting calves were recorded. Results show much similarity between groups in terms of pregnancy rates, gestation length and calves’ weight. Nonetheless, several differences on hormonal levels were noted between recipients carrying AI embryos especially when compared to BSA. Some calving issues and neonatal mortality were observed in both IVP groups. In conclusion, most of the parameters studied were similar between both types of IVP derived embryos and the in vivo-derived embryos, suggesting that the IVP technology used was efficient enough for the safe production of calves.

中文翻译:

人工授精与胚胎移植奶牛的生殖结果和内分泌特征。

越来越多地使用体外胚胎生产(IVP),接着进行胚胎移植(ET),以及冷冻保存胚胎,这引起了人们对可能改变的怀孕率,产犊甚至新生儿死亡率的担忧。这些改变的假设之一是IVP的当前培养条件。为了更好地模仿生理环境,用雌性生殖液(RF)作为培养基的补充物生产了胚胎,并向另一组胚胎补充了牛血清白蛋白(BSA)作为体外对照。将胚胎冷冻保存并转移,与此同时,同时包括具有与用于IVP的相同公牛的体内对照(人工授精,AI)。记录妊娠率,受者荷尔蒙水平,分娩和产犊的概况。结果显示,在妊娠率,妊娠长度和犊牛体重方面,各组之间非常相似。尽管如此,在携带AI胚胎的受者之间,荷尔蒙水平存在一些差异,特别是与BSA相比。在两个IVP组中均观察到一些产犊问题和新生儿死亡率。总之,研究的大多数参数在两种类型的IVP来源的胚胎和体内来源的胚胎之间都是相似的,这表明所使用的IVP技术足以有效地安全生产犊牛。
更新日期:2020-08-06
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