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Comparative transcriptomic analysis to identify brassinosteroid response genes.
Plant Physiology ( IF 7.4 ) Pub Date : 2020-10-01 , DOI: 10.1104/pp.20.00386
Xiaolei Liu 1 , Hongxing Yang 2 , Yuan Wang 3 , Zhaohai Zhu 4 , Wei Zhang 4 , Jianming Li 1, 5, 6
Affiliation  

Brassinosteroids (BRs) are plant growth-promoting steroid hormones. BRs affect plant growth by regulating panels of downstream genes. Much effort has been made to establish BR-regulated gene expression networks, but there is little overlap among published expression networks. In this study, we built an optimal BR-regulated gene expression network using the model plant Arabidopisis (Arabidopisis thaliana). Seven- and 24-d-old seedlings of the constitutive photomorphogenesis and dwarfism mutant and brassinosteroid-insensitive 1-701 (bri1-701) BRI1-like receptor genes1 (brl1) brl3 triple mutant seedlings were treated with brassinolide and RNA sequencing (RNA-seq) was used to detect differentially expressed genes. Using this approach, we generated a transcriptomic database of 4,498 differentially expressed genes and identified 110 transcription factors that specifically respond to BR at different stages. We also found that, among the identified BR-responsive transcription factors, ABSCISIC ACID-INSENSlTIVE4 (ABI4), an ethylene response factor transcription factor, inhibits BR-regulated growth. Compared to wild-type plants, the abi4-102 mutant was less sensitive to brassinazole and more sensitive to BR. Next, we performed a chromatin immunoprecipitation followed by high-throughput sequencing assay and established that ABI4 binds directly to the BRI1-associated receptor kinase1 promoter and inhibits transcription. These results provide insight into BR-responsive gene functions in regulating plant growth at different stages and may serve as a basis for predicting gene function, selecting candidate genes, and improving the understanding of BR regulatory pathways.



中文翻译:

比较转录组分析以确定油菜素类固醇反应基因。

油菜素类固醇(BR)是促进植物生长的类固醇激素。BR 通过调节下游基因组来影响植物生长。人们为建立 BR 调控的基因表达网络做出了很多努力,但已发表的表达网络之间几乎没有重叠。在本研究中,我们利用模式植物拟南芥( Arabidopisis thaliana)构建了最佳的BR调控基因表达网络。用油菜内酯和RNA测序RNA- _ _ _ _ seq)用于检测差异表达基因。利用这种方法,我们生成了 4,498 个差异表达基因的转录组数据库,并鉴定了 110 个在不同阶段对 BR 特异性反应的转录因子。我们还发现,在已鉴定的 BR 响应转录因子中,ABSCISIC ACID-INSENSlTIVE4 (ABI4)(一种乙烯响应因子转录因子)可抑制 BR 调节的生长。与野生型植物相比,abi4-102突变体对芸苔素不太敏感,而对BR更敏感。接下来,我们进行了染色质免疫沉淀,然后进行高通量测序测定,并确定 ABI4 直接与BRI1 相关受体激酶 1启动子结合并抑制转录。这些结果提供了对BR响应基因在不同阶段调节植物生长的功能的深入了解,并可作为预测基因功能、选择候选基因和提高对BR调控途径的理解的基础。

更新日期:2020-10-06
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