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Ginsenoside Rb1 attenuates microglia activation to improve spinal cord injury via microRNA-130b-5p/TLR4/NF-κB axis.
Journal of Cellular Physiology ( IF 5.6 ) Pub Date : 2020-08-06 , DOI: 10.1002/jcp.30001
Dan Wang 1 , Shixin Zhao 1 , Junwei Pan 1 , Zhen Wang 1 , Yu Li 1 , Xiaoxiao Xu 1 , Jiahao Yang 1 , Xi Zhang 1 , Yisheng Wang 1 , Ming Liu 1
Affiliation  

Ginsenoside Rb1 (GRb1), a major ingredient of ginseng, has been found to be a potential protective agent in spinal cord injury (SCI) and in activated microglia‐induced neuronal injury. This study discovered that GRb1 could facilitate miR‐130b‐5p expression in SCI rats and Toll‐like receptor 4 (TLR4; a crucial player in inflammation) was a potential target of miR‐130b‐5p. Hence, we further investigated whether GRb1 could relieve SCI by reducing microglia‐mediated inflammatory responses and neuronal injury via miR‐130b‐5p/TLR4 pathways. The results showed that GRb1 alleviated SCI through inhibiting neuronal apoptosis and proinflammatory factor expression via increasing miR‐130b‐5p.GRb1 weakened the damage of activated microglia to neurons through upregulating miR‐130b‐5p. miR‐130b‐5p attenuated activated microglia‐induced neuron injury via targeting TLR4. GRb1 inactivated TLR4/nuclear factor‐κB (NF‐κB) activation and inhibited proinflammatory cytokine secretion by increasing miR‐130b‐5p in activated microglia. As a conclusion, GRb1 alleviated SCI through reducing activated microglia‐induced neuronal injury via miR‐130b‐5p/TLR4/NF‐κB axis, providing a deep insight into the molecular basis of GRb1 in the treatment of SCI.

中文翻译:

人参皂苷 Rb1 通过 microRNA-130b-5p/TLR4/NF-κB 轴减弱小胶质细胞活化以改善脊髓损伤。

人参的主要成分人参皂苷 Rb1 (GRb1) 已被发现是脊髓损伤 (SCI) 和活化小胶质细胞诱导的神经元损伤的潜在保护剂。该研究发现 GRb1 可以促进 SCI 大鼠中 miR-130b-5p 的表达,Toll 样受体 4(TLR4;炎症的关键参与者)是 miR-130b-5p 的潜在靶标。因此,我们进一步研究了 GRb1 是否可以通过 miR-130b-5p/TLR4 通路减少小胶质细胞介导的炎症反应和神经元损伤来缓解 SCI。结果表明,GRb1通过增加miR-130b-5p抑制神经元凋亡和促炎因子表达来减轻SCI。GRb1通过上调miR-130b-5p减弱活化小胶质细胞对神经元的损伤。miR-130b-5p 通过靶向 TLR4 减轻激活的小胶质细胞诱导的神经元损伤。GRb1 通过增加活化小胶质细胞中的 miR-130b-5p 来灭活 TLR4/核因子-κB(NF-κB)活化并抑制促炎细胞因子的分泌。总之,GRb1 通过 miR-130b-5p/TLR4/NF-κB 轴减少激活的小胶质细胞诱导的神经元损伤来减轻 SCI,为深入了解 GRb1 治疗 SCI 的分子基础提供了依据。
更新日期:2020-08-06
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