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Harnessing electrospun nanofibers to recapitulate hierarchical fibrous structures of meniscus.
Journal of Biomedical Materials Research Part B: Applied Biomaterials ( IF 3.4 ) Pub Date : 2020-08-05 , DOI: 10.1002/jbm.b.34692
Xiaoyu Wang 1 , Jingjing Zhu 1 , Binbin Sun 1 , Qiu Jin 1 , Haiyan Li 1 , Changlei Xia 2 , Hongsheng Wang 1 , Xiumei Mo 1 , Jinglei Wu 1, 3
Affiliation  

The meniscus has complex biomechanical functions endowed by the hierarchical fibrous structure of its extracellular matrix (ECM), which plays a central role in protecting the knee joint. However, it is challenging to recapitulate the ECM structure of the meniscus. Herein, we used electrospinning to prepare various scaffolds with distinct nanofibrous structures to approximate that of the heterogeneous ultrastructure of meniscus ECM. Our results showed that adjusting the deposition manner of nanofibers during electrospinning could effectively manipulate the architectures of resulting scaffolds. This approach led to electrospun scaffolds with random or aligned fibrous structures that reassemble the surface or superficial layers of the meniscus. We also showed that assembly of electrospun nanofibers into yarn‐like configurations replicates the circumferentially aligned fibrous bundles of the inner part of the meniscus. These scaffolds exhibited distinct fibrous anisotropies and mechanical properties. In vitro studies indicated good cytocompatibility of scaffolds, especially, the yarn scaffold supported considerable meniscus cell infiltration. The meniscus cells, in turn, enhanced the mechanical properties of scaffolds. Taken together, these data imply that electrospun scaffolds may have potential in enhancing meniscus repair and regeneration.

中文翻译:

利用电纺纳米纤维来概括半月板的分层纤维结构。

半月板具有复杂的生物力学功能,由其细胞外基质 (ECM) 的分层纤维结构赋予,在保护膝关节方面发挥着核心作用。然而,概括半月板的 ECM 结构具有挑战性。在这里,我们使用静电纺丝制备了具有不同纳米纤维结构的各种支架,以近似半月板 ECM 的异质超微结构。我们的结果表明,在静电纺丝过程中调整纳米纤维的沉积方式可以有效地控制所得支架的结构。这种方法导致静电纺丝支架具有随机或对齐的纤维结构,可重新组装半月板的表面或浅层。我们还表明,将电纺纳米纤维组装成纱线状结构复制了弯月面内部的周向排列的纤维束。这些支架表现出明显的纤维各向异性和机械性能。体外研究表明支架具有良好的细胞相容性,特别是纱线支架支持大量半月板细胞浸润。反过来,半月板细胞增强了支架的机械性能。总之,这些数据表明静电纺丝支架可能具有增强半月板修复和再生的潜力。纱线支架支持大量半月板细胞浸润。反过来,半月板细胞增强了支架的机械性能。总之,这些数据表明静电纺丝支架可能具有增强半月板修复和再生的潜力。纱线支架支持大量半月板细胞浸润。反过来,半月板细胞增强了支架的机械性能。总之,这些数据表明静电纺丝支架可能具有增强半月板修复和再生的潜力。
更新日期:2020-08-05
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