Wound healing requires well-coordinated events including hemostasis, inflammation, proliferation, and remodeling. Delays in any of these stages leads to chronic wounds, infections, and hypertrophic scarring. Burn wounds are particularly problematic, and may require intervention to ensure timely progression to reduce morbidity and mortality. To accelerate burn wound healing, Platelet-Rich Plasma (PRP)¹ can be of value, since platelets release growth factor proteins and inorganic polyphosphates (polyP) that may be integral to wound healing. We used polyP-depleted keratinocyte (HaCaT) and fibroblast cell culture models to determine cell proliferation and scratch-wound repair to determine if polyP, platelet lysate, or combined treatment could accelerate wound healing. While polyP and PRP significantly reduced the open scratch-wound area in fibroblasts and keratinocytes, polyP had no effect on keratinocyte or fibroblast proliferation. PRP was also evaluated as a treatment in a murine model of full thickness wound healing in vivo, including a treatment in which PRP was supplemented with purified polyP. PRP induced significantly more rapid re-epithelialization by Day 3. Pure polyP enhanced the effects of PRP on epithelial tongues, which were significantly elongated in the PRP + high-dose polyP treatment groups compared to PRP alone. Thus, PRP and polyP may serve as an effective therapeutic combination for treating wounds.

伤口愈合需要协调一致的事件，包括止血，炎症，增殖和重塑。这些阶段的任何延迟都会导致慢性伤口，感染和肥厚性瘢痕形成。烧伤伤口特别成问题，可能需要干预以确保及时进展，以降低发病率和死亡率。为了加速烧伤创面愈合，富血小板血浆（PRP）¹由于血小板释放的生长因子蛋白和无机多磷酸盐（polyP）可能是伤口愈合必不可少的，因此具有重要意义。我们使用了贫聚角蛋白细胞（HaCaT）和成纤维细胞培养模型来确定细胞增殖和刮伤修复，以确定polyP，血小板裂解液或联合治疗是否可以加速伤口愈合。尽管polyP和PRP显着减少了成纤维细胞和角质形成细胞中开放的划痕伤口区域，但polyP对角质形成细胞或成纤维细胞增殖没有影响。还评估了PRP作为在体内全层伤口愈合的鼠模型中的治疗方法，包括在PRP中添加纯化的polyP的治疗。到第3天，PRP诱导的上皮再生明显更快。纯polyP增强了PRP对上皮舌头的作用，与单独使用PRP相比，在PRP +大剂量polyP治疗组中，这种作用明显延长。因此，PRP和polyP可以用作治疗伤口的有效治疗组合。