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Electrical Stimulation Evokes Rotational Behavior In Tandem with Exocytotic-like Increases in Dopamine Measured by In Vivo Intracerebral Microdialysis.
Journal of Neuroscience Methods ( IF 3 ) Pub Date : 2020-08-06 , DOI: 10.1016/j.jneumeth.2020.108894
Alice H Grant 1 , Mabel A Terminel 1 , Jeremiah Ramos 1 , Luisa F Alatorre 1 , Edward Castañeda 2
Affiliation  

Background

Electrical Stimulation is a traditional tool in neuroscience and is commonly used in vivo to evoke behavior and in vitro to study neural mechanisms. In vivo intracerebral microdialysis, also a traditional technique, is used to assay neurotransmitter release. However, the combination of these techniques is highly limited to studies using anesthetized animals; therefore, evoking and measuring exocytotic neurotransmitter release in awake models is lacking. Combining these techniques in an awake animal preparation is presented here with evidence to support the mechanistic action of electrical stimulation in vivo.

New Methods

This report presents converging evidence to validate the combination of intracerebral electrical stimulation with microdialysis as a novel procedure to study exocytotic-like dopamine release in behaving animals.

Results

It is shown that electrical stimulation of the medial forebrain bundle can be used to evoke frequency- and intensity-dependent exocytotic-like dopamine overflow and rotational behavior that are sensitive to Na+ channel blockade and Ca++ availability.

Comparison with Existing Methods

Studies using modern techniques to evoke neurotransmitter release, combined with in vivo intracerebral microdialysis, and measured behavioral output are scarce. In contrast, commonly used pharmacological methods often are less precise and inefficient to evoke exocytotic dopamine release and behavior. Here we demonstrate, the combination of in vivo intracerebral microdialysis with electrical stimulation as a simple approach to simultaneously assess physiologically relevant neurotransmitter ‘release’ and behavior.

Conclusions

Research that aims to understand how dopamine neurotransmission is altered in behavioral disorders can utilize this innovative combination of electrical stimulation with in vivo intracerebral microdialysis.



中文翻译:

电刺激引起旋转行为,同时通过体内微透析测量多巴胺的胞吐样增加。

背景

电刺激是神经科学中的传统工具,通常用于体内诱发行为和体外研究神经机制。体内脑微透析,也是一种传统技术,用于测定神经递质释放。然而,这些技术的组合仅限于使用麻醉动物的研究;因此,缺乏唤醒和测量清醒模型中胞吐神经递质释放的方法。将这些技术结合在清醒的动物制剂中,这里提供了支持体内电刺激机械作用的证据。

新方法

本报告提供了汇总证据,以验证脑内电刺激与微透析的组合作为研究行为动物中胞吐样多巴胺释放的新程序。

结果

结果表明,内侧前脑束的电刺激可用于诱发频率和强度依赖性胞吐作用样多巴胺溢出和旋转行为,这些行为对 Na +通道阻滞和 Ca ++可用性敏感。

与现有方法的比较

使用现代技术唤起神经递质释放,结合体内脑内微透析和测量的行为输出的研究很少。相比之下,常用的药理学方法通常不太精确且效率低下,无法激发胞吐多巴胺的释放和行为。在这里,我们展示了将体内脑微透析与电刺激相结合作为一种简单的方法来同时评估生理相关的神经递质“释放”和行为。

结论

旨在了解行为障碍中多巴胺神经传递如何改变的研究可以利用这种电刺激与体内脑微透析的创新组合。

更新日期:2020-09-02
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