Clubroot disease, caused by Plasmodiophora brassicae, is one of the most destructive soil-borne diseases in cruciferous crops worldwide. New strategies are urgently needed to control this disease, as no effective disease-resistant varieties or chemical control agents exist. Previously, we found that the incidence rate and disease index of clubroot in oilseed rape decreased by 50 and 40%, respectively, when oilseed rape was planted after soybean. In order to understand how different rotation patterns affect the occurrence of clubroot in oilseed rape, high-throughput sequencing was used to analyze the rhizosphere microbial community of oilseed rape planted after leguminous (soybean, clover), gramineous (rice, maize) and cruciferous (oilseed rape, Chinese cabbage) crops. Results showed that planting soybeans before oilseed rape significantly increased the population density of microbes that could inhibit P. brassicae (e.g., Sphingomonas, Bacillus, Streptomyces and Trichoderma). Conversely, consecutive cultivation of cruciferous crops significantly accumulated plant pathogens, including P. brassicae, Olpidium and Colletotrichum (P<0.05). These results will help to develop the most effective rotation pattern for reducing clubroot damage.

根瘤病引起的根瘤病，是全世界十字花科作物中最具破坏性的土传疾病之一。由于不存在有效的抗病品种或化学防治剂，因此迫切需要新的策略来控制这种疾病。以前，我们发现，在大豆后种植油菜时，油菜的根瘤病发生率和疾病指数分别降低了50％和40％。为了了解不同的轮作方式如何影响油菜油菜根的发生，采用高通量测序方法分析了豆科（大豆，三叶草），禾本科（大米，玉米）和十字花科（油菜籽）后种植的油菜的根际微生物群落。油菜，大白菜）作物。芸苔假单胞菌（例如鞘氨醇单胞菌，芽孢杆菌，链霉菌和木霉属）。相反，十字花科作物的连续栽培显着地积累了植物病原体，包括芸苔假单胞菌，臭大头菜和炭疽菌（P <0.05）。这些结果将有助于开发出最有效的旋转方式，以减少球根的损伤。