This study presents evaluation of the possible mechanisms of interaction between the antiepileptic drug lamotrigine (LMT) and single- and double-stranded DNA (ssDNA and dsDNA, respectively). These interactions were studied in phosphate-buffered saline (PBS) at physiological pH 7.4 by cyclic voltammetry (CV) and square wave voltammetry (SWV) using a glassy carbon electrode (GCE) in a bulk incubated solution. The addition of both types of DNA to LMT solution decreased peak currents and led to a negative shift in peak potentials, thus indicating the dominance of electrostatic interactions. UV–Vis absorption spectroscopy was also used to assess the interaction between ds/ssDNA and LMT. The data obtained from spectroscopic analysis confirmed that electrostatic interaction is the predominant interaction between LMT and both types of DNA. The calculated binding constants for LMT-dsDNA and LMT-ssDNA complexes as determined by SWV were 6.46 × 10⁵ and 1.81 × 10⁶, respectively, while the values obtained from UV–Vis spectroscopy were 6.93 × 10⁵ and 1.19 × 10⁶, respectively. The obtained results indicated a higher affinity of LMT for ssDNA than for dsDNA.

这项研究提出了抗癫痫药拉莫三嗪（LMT）与单链和双链DNA（分别为ssDNA和dsDNA）之间相互作用的可能机制的评估。在生理pH为7.4的磷酸盐缓冲盐水（PBS）中，通过循环伏安法（CV）和方波伏安法（SWV）使用玻璃碳电极（GCE）在大量孵育溶液中研究了这些相互作用。在LMT溶液中添加两种类型的DNA会降低峰值电流，并导致峰值电势发生负向偏移，从而表明静电相互作用占主导地位。紫外可见吸收光谱法也用于评估ds / ssDNA与LMT之间的相互作用。从光谱分析获得的数据证实，静电相互作用是LMT与两种DNA之间的主要相互作用。^分别为5和1.81×10 ⁶，而从紫外可见光谱法获得的值分别为6.93×10 ⁵和1.19×10 ⁶。获得的结果表明，LMT对ssDNA的亲和力高于对dsDNA的亲和力。