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Dual targeting of TatA points to a chloroplast-like Tat pathway in plant mitochondria.
Biochimica et Biophysica Acta (BBA) - Molecular Cell Research ( IF 5.1 ) Pub Date : 2020-08-06 , DOI: 10.1016/j.bbamcr.2020.118816
Bationa Bennewitz 1 , Mayank Sharma 1 , Franzisca Tannert 1 , Ralf Bernd Klösgen 1
Affiliation  

The biogenesis of membrane-bound electron transport chains requires membrane translocation pathways for folded proteins carrying complex cofactors, like the Rieske Fe/S proteins. Two independent systems were developed during evolution, namely the Twin-arginine translocation (Tat) pathway, which is present in bacteria and chloroplasts, and the Bcs1 pathway found in mitochondria of yeast and mammals. Mitochondria of plants carry a Tat-like pathway which was hypothesized to operate with only two subunits, a TatB-like protein and a TatC homolog (OrfX), but lacking TatA. Here we show that the nuclearly encoded TatA from pea has dual targeting properties, i.e., it can be imported into both, chloroplasts and mitochondria. Dual targeting of TatA was observed with in organello experiments employing chloroplasts and mitochondria isolated from pea as well as after transient expression of suitable reporter constructs in leaf tissue from pea and Nicotiana benthamiana. The extent of transport of these constructs into mitochondria of transiently transformed leaf cells was relatively low, causing a demand for highly sensitive methods to be detected, like the sasplitGFP approach. Yet, the dual import of TatA into mitochondria and chloroplasts observed here points to a common mechanism of Tat transport for folded proteins within both endosymbiotic organelles in plants.



中文翻译:

TatA的双重靶向指向植物线粒体中的叶绿体样Tat途径。

膜结合电子传输链的生物发生需要带有复杂辅因子的折叠蛋白(如Rieske Fe / S蛋白)的膜移位途径。在进化过程中开发了两个独立的系统,即存在于细菌和叶绿体中的双精氨酸易位(Tat)途径,以及存在于酵母和哺乳动物线粒体中的Bcs1途径。植物的线粒体携带着一种Tat样途径,该途径被假定仅与两个亚基一起运行,即一个TatB样蛋白和一个TatC同源物(OrfX),但缺少TatA。在这里,我们显示了豌豆的核编码TatA具有双重靶向特性,即可以导入叶绿体和线粒体中。在器官中观察到TatA的双重靶向利用从豌豆分离的叶绿体和线粒体进行的实验,以及在豌豆和烟草的叶片组织中瞬时表达合适的报告基因构建后的实验。这些构建体转运到瞬时转化的叶细胞的线粒体中的程度相对较低,导致需要检测高度敏感的方法,例如sa splitGFP方法。然而,此处观察到的TatA双重输入线粒体和叶绿体中,表明了Tat转运植物中两个内共生细胞器内折叠蛋白的共同机制。

更新日期:2020-08-22
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