Anaerobe ( IF 2.3 ) Pub Date : 2020-08-06 , DOI: 10.1016/j.anaerobe.2020.102240 Nurver Ulger Toprak 1 , Oncu Akgul 1 , József Sóki 2 , Guner Soyletir 1 , Elisabeth Nagy 2 ,
Penicillins, can be used in treatment of infections due to Prevotella species if they are susceptible to penicillin. Early and accurate preliminary detection of β-lactamase-producing isolates is crucial for treatment of infection. The aim of this study was to determine β-lactamase-producing Prevotella species by MALDI-TOF MS and screen them for the presence of cfxA gene, responsible for β-lactamase production. A total of 500 clinically relevant Prevotella isolates, collected from 13 countries for the previous European antibiotic resistance surveillance study, were tested. Susceptibility testing was performed against ampicillin and ampicillin/sulbactam by Etest methodology. EUCAST guidelines were used for susceptibility interpretations; the isolates with MIC value ≤ 0.5 for ampicillin were considered susceptible and >2 resistant. All Prevotella isolates, were tested for detection of β-lactamase activity by MALDI-TOF MS (Vitek® MS Research Use Only) system and the presence of the cfxA gene by PCR method. The susceptibility levels of the isolates to ampicillin/sulbactam and ampicillin were 99.6% and 43.4%, respectively. A total 59% of isolates presented β-lactamase activity and 60.8% were cfxA gene positive. Both these tests were positive for isolates in the resistant category. Additionally, >95% of the isolates (n = 65) which ampicillin MIC values ranged from >0.5 μg/mL to 2 μg/ml displayed β-lactamase activity. We also found that the MALDI-TOF MS-based β-lactamase assay delivers results in 2 h. We found a high concordance between the MALDI-TOF MS β-lactamase results in terms of cfxA β-lactamase gene presence. MALDI-TOF MS may serve as a simple and efficient alternative method of the existing phenotypic and PCR-based methods.
中文翻译:
通过MALDI-TOF MS方法检测临床普氏杆菌中β-内酰胺酶的产生。
如果青霉素易受青霉素感染,则可用于治疗因普氏杆菌属引起的感染。早期和准确地初步检测产生β-内酰胺酶的分离株对于治疗感染至关重要。这项研究的目的是通过MALDI-TOF MS确定产生β-内酰胺酶的Prevotella菌种,并筛选它们是否存在cfx A基因,该基因负责产生β-内酰胺酶。共有500种临床相关的普罗沃氏菌测试了从13个国家/地区收集的分离株,用于先前的欧洲抗生素耐药性监测研究。通过Etest方法对氨苄西林和氨苄西林/舒巴坦进行药敏试验。使用EUCAST指南进行磁化率解释;氨苄青霉素的MIC值≤0.5的分离株被认为易感且抗药性> 2。通过MALDI-TOF MS(仅用于Vitek®MS研究用)系统测试所有Prevotella分离株的β-内酰胺酶活性,并通过PCR方法检测cfx A基因的存在。分离株对氨苄西林/舒巴坦和氨苄西林的敏感性水平分别为99.6%和43.4%。共有59%的分离物表现出β-内酰胺酶活性,其中60.8%的是cfx基因阳性。这两个测试对耐药类别的分离株均呈阳性。另外,氨苄青霉素MIC值范围从> 0.5μg/ mL到2μg/ ml的> 95%分离株(n = 65)表现出β-内酰胺酶活性。我们还发现,基于MALDI-TOF MS的β-内酰胺酶测定法可在2小时内提供结果。我们发现在cfx Aβ-内酰胺酶基因存在方面,MALDI-TOF MSβ-内酰胺酶结果之间存在高度一致性。MALDI-TOF MS可以作为现有基于表型和PCR的方法的一种简单有效的替代方法。