Only few studies examined the prognostic effect of tumor budding in esophageal adenocarcinomas so far. However, different quantification approaches were used, so results cannot be directly compared. Recently, the International Tumor Budding Consensus Conference (ITBCC) published consensus criteria for the evaluation of tumor budding in colorectal cancer, which we applied in our study. Hematoxylin and eosin (H&E) and cytokeratin (AE1/AE3) stained whole tissue slides of 104 resected esophageal adenocarcinomas were evaluated. The mean count of tumor buds was analyzed in one high power field according to the ITBCC criteria and assigned to budding groups Bd1-3. Tumor budding was significantly associated with a worse overall survival. Regardless of the quantification approach, an increased number of tumor buds was significantly associated with reduced overall survival (OS) (H&E: HR = 1.05 (95% CI 1.029–1.073), p < 0.001; cytokeratin: HR = 1.073 (95% CI 1.045–1.101), p < 0.001). In multivariable analysis tumor budding according to ITBCC criteria on H&E stained slides was an independent prognostic factor. Tumor budding, according to ITBCC criteria, is an independent prognostic factor in resected esophageal adenocarcinoma. Prospective studies using ITBCC criteria are useful in the near future to validate our results.

迄今为止，只有很少的研究检查了肿瘤萌发在食管腺癌中的预后效果。但是，由于使用了不同的量化方法，因此无法直接比较结果。最近，国际肿瘤萌芽共识会议（ITBCC）发布了用于评估结直肠癌肿瘤萌发的共识标准，该标准已在我们的研究中应用。苏木精和曙红（H＆E）和细胞角蛋白（AE1 / AE3）染色的104例切除的食管腺癌的整个组织切片进行了评估。根据ITBCC标准，在一个高倍视野中分析了肿瘤芽的平均数量，并将其分配给萌芽组Bd1-3。肿瘤出芽与较差的总体存活率显着相关。不管采用哪种量化方法，p  <0.001；细胞角蛋白：HR = 1.073（95％CI 1.045-1.011），p  <0.001）。在多变量分析中，根据ITBCC标准对H＆E染色载玻片上的肿瘤出芽是独立的预后因素。根据ITBCC标准，肿瘤发芽是食管腺癌切除的独立预后因素。使用ITBCC标准的前瞻性研究在不久的将来对验证我们的结果很有用。