Acinetobacter baumannii (A. baumannii) which is one of the most problematic causative agents of nosocomial infections, worldwide, can severely cause morbidity and mortality within the healthcare community and present a particular threat to intensive care unit patients. The infection caused by these bacteria strains is a major cause for concern among healthcare professionals and is prompting changes in both antibiotic regiments and hospital disinfection techniques. Different protocols have been employed for the identification of samples or hospital equipment, colonized by A. baumannii, so that appropriate decontamination procedures can be carried out. In this work, we introduced a new diagnostic test for A. baumannii with a molecularly imprinted polymer (MIP). The MIP was carried out on the surface of the glassy carbon electrode applying the electropolymerization of the dopamine monomer with the template of A. baumannii. The electrochemical techniques, such as cyclic voltammetry and impedance spectroscopy, were performed in order to characterize the NIP and MIP modified electrode in the hexacyanoferrate redox system as an electrochemical probe. The sensor exhibited a linear range of 10²–10⁷ CFU L⁻¹ for detecting A. baumannii with a detection limit of 30 CFU L⁻¹. This sensor was successfully used to test the recovery of A. baumannii in human blood serum samples. According to our knowledge, this is the first report on a MIP sensor to detect A. baumannii.

中文翻译：

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基于聚多巴胺分子印迹聚合物的新型电化学传感器用于鲍曼不动杆菌的灵敏和选择性检测

在世界范围内，鲍曼不动杆菌（鲍曼不动杆菌）是医院感染中最成问题的病原体之一，它可以严重引起医疗保健界内的发病率和死亡率，并且对重症监护病房患者构成特别的威胁。这些细菌菌株引起的感染是引起医疗保健专业人员关注的主要原因，并促使抗生素治疗方案和医院消毒技术发生变化。已经采用了不同的方案来鉴定鲍曼不动杆菌所定殖的样品或医院设备，以便可以进行适当的净化程序。在这项工作中，我们引入了一种针对鲍曼不动杆菌的新诊断测试带有分子印迹聚合物（MIP）。在多巴胺单体与鲍曼不动杆菌的模板上进行电聚合，在玻璃碳电极的表面上进行MIP 。进行电化学技术，例如循环伏安法和阻抗谱法，以将六氰合铁酸盐氧化还原系统中的NIP和MIP修饰电极表征为电化学探针。该传感器的线性范围为10 ² –10 ⁷  CFU L ^-1，用于检测鲍曼不动杆菌，检测极限为30 CFU L ^-1。该传感器已成功用于测试鲍曼不动杆菌的回收率在人血清样本中。据我们所知，这是有关检测鲍曼不动杆菌的MIP传感器的第一份报告。