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Molecular characterization of a new bipartite begomovirus that infects okra plants in guerrero, mexico
Journal of Plant Diseases and Protection ( IF 2 ) Pub Date : 2020-08-06 , DOI: 10.1007/s41348-020-00356-4
Ernestina Valadez-Moctezuma , Samir Samah , Lily Xochilt Zelaya-Molina , Joaquín Bernardo Díaz-Rivera

In Mexico, okra (Abelmoschus esculentus L.) is grown mainly for export, but its production is affected by various pests and diseases. Plants with mosaic and chlorotic mottling symptoms were collected in Iguala, Mexico. The transmission tests revealed that the disease was not transmitted neither by seed nor by mechanical means. However, it was transmitted by grafting and by insect vectors. The entire nucleotide sequences of the DNA-A component of three isolates were obtained by PCR and sequencing; all of them were bipartite begomoviruses. The composition of the DNA-A component consists of five open reading frames (ORFs), one in the sense orientation (AV1) and four in antisense orientation (AC1, AC2, AC3 and AC4), in addition to the common region (CR). Sequence comparison and demarcation analyses revealed the presence of a new strain of Okra yellow mosaic Mexico virus—[Mexico:Isolate 13–14:2010], and a new species not previously reported and tentatively named Okra mottle Mexico virus, OMoMV—[Mexico:2005]. The phylogenetic analysis placed the new species in the clade that contains Chino del tomate virus. Recombination analyses suggest that this virus has a putative recombinant event of 93 bases, which may have originated across the exchange of genomic segments in the ORF AV1 + CR region from Chino del tomate virus, Okra yellow mosaic Mexico virus and Corchorus yellow spot virus.



中文翻译:

新型双倍性begomovirus的分子特征,其感染墨西哥格雷罗州的秋葵植物

在墨西哥,秋葵(Abelmoschus esculentusL.)主要用于出口,但其生产受到各种病虫害的影响。在墨西哥的伊瓜拉收集了有花叶病和绿藻斑纹症状的植物。传播测试表明,该疾病既没有通过种子也没有通过机械手段传播。但是,它是通过嫁接和昆虫媒介传播的。通过PCR和测序获得了三种分离物的DNA-A组分的完整核苷酸序列。它们都是二倍体begomoviruses。除了公共区域(CR),DNA-A成分的组成还包括五个开放阅读框(ORF),一个为有义方向(AV1),四个为反义方向(AC1,AC2,AC3和AC4)。 。序列比较和分界分析揭示了一种新菌株的存在。黄秋葵黄花叶病墨西哥病毒-[Mexico:Isolate 13-14:2010],以及以前未曾报道过的新物种,并暂定名为Okra斑驳墨西哥病毒OMoMV- [Mexico:2005]。系统发育分析将新物种放入了含有奇诺菌的进化枝中。重组分析表明该病毒具有93个碱基的推定重组事件,该事件可能起源于通吃奇诺del tomate病毒黄秋葵黄花叶墨西哥病毒Corchorus黄斑病毒的ORF AV1 + CR区的基因组片段的交换。

更新日期:2020-08-06
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