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Effects of Fe(II) on anammox community activity and physiologic response
Frontiers of Environmental Science & Engineering ( IF 6.4 ) Pub Date : 2020-07-30 , DOI: 10.1007/s11783-020-1299-9
Jing Ding , Wanyi Seow , Jizhong Zhou , Raymond Jianxiong Zeng , Jun Gu , Yan Zhou

Though there are many literatures studying the effects of iron on anammox process, these studies only focus on the reactor performance and/or the microbial community changes, the detailed effects and mechanisms of Fe(II) on anammox bacterial activity and physiology have not been explored. In this study, four Fe(II) concentrations (0.03,0.09,0.12 and 0.75mmol/L) were employed into the enriched anammox culture. The enhancement and inhibition effects of Fe(II) on anammox process and bacterial physiology were investigated. It was discovered that the anammox process and bacterial growth were enhanced by 0.09 and 0.12 mmol/L Fe(II), in which the 0.12 mmol/L Fe(II) had advantage in stimulating the total anammox activity and bacterial abundance, while 0.09 mmol/L Fe (II) enhanced the relative anammox activity better. The anammox activity could be inhibited by 0.75 mmol/L Fe(II) immediately, while the inhibition was recoverable. Both 0.09 and 0.12 mmol/L Fe(II) induced more genes being expressed, while didn’t show a stimulation on the relative expression level of functional genes. And anammox bacteria showed a stress response to detoxify the Fe inhibition once inhibited by 0.75 mmol/L Fe(II). This study provides more information about physiologic response of anammox bacteria to external influence (enhancement and inhibition), and may also instruct the future application of anammox process in treating various sources of wastewater (containing external disturbances such as heavy metals) and/or different treatment strategies (e.g. from side-stream to main-stream).



中文翻译:

Fe(II)对厌氧氨氧化菌群落活性和生理反应的影响

尽管有许多文献研究铁对厌氧菌过程的影响,但这些研究仅集中在反应器性能和/或微生物群落变化上,尚未探讨铁(II)对厌氧菌活性和生理的详细作用和机理。 。在这项研究中,四个Fe(II)浓度(0.03、0.09、0.12和0.75mmol / L)被用于富集的厌氧氨氧化菌培养物中。研究了Fe(II)对厌氧氨氧化过程和细菌生理的增强和抑制作用。发现0.09和0.12 mmol / L Fe(II)可促进厌氧氨氧化过程和细菌生长,其中0.12 mmol / L Fe(II)有利于刺激总厌氧菌活性和细菌丰度,而0.09 mmol / L Fe(II)更好地增强了相对厌氧氨氧化活性。0.75 mmol / L Fe(II)可以立即抑制厌氧氨氧化活性,而这种抑制作用是可以恢复的。0.09和0.12 mmol / L Fe(II)都诱导更多的基因表达,而对功能基因的相对表达水平没有刺激。厌氧性厌氧细菌一旦受到0.75 mmol / L Fe(II)的抑制,便表现出应激反应以解毒Fe抑制。这项研究提供了有关厌氧氨氧化细菌对外部影响(增强和抑制)的生理反应的更多信息,并且还可能指导厌氧氨氧化工艺在处理各种废水(包含外部干扰,例如重金属)和/或不同处理方法方面的未来应用。策略(例如从侧流到主流)。12 mmol / L Fe(II)诱导更多的基因表达,而对功能基因的相对表达水平没有刺激。厌氧性厌氧细菌一旦受到0.75 mmol / L Fe(II)的抑制,便表现出应激反应以解毒Fe抑制。这项研究提供了有关厌氧氨氧化细菌对外部影响(增强和抑制)的生理反应的更多信息,并且还可能指导厌氧氨氧化工艺在处理各种废水(包含外部干扰,例如重金属)和/或不同处理方法方面的未来应用。策略(例如从侧流到主流)。12 mmol / L Fe(II)诱导更多的基因表达,而对功能基因的相对表达水平没有刺激。厌氧性厌氧细菌一旦受到0.75 mmol / L Fe(II)的抑制,便表现出应激反应以解毒Fe抑制。这项研究提供了有关厌氧氨氧化细菌对外部影响(增强和抑制)的生理反应的更多信息,并且还可能指导厌氧氨氧化工艺在处理各种废水(包含外部干扰,例如重金属)和/或不同处理方法方面的未来应用。策略(例如从侧流到主流)。75 mmol / L的Fe(II)。这项研究提供了有关厌氧氨氧化细菌对外部影响(增强和抑制)的生理反应的更多信息,并且还可能指导厌氧氨氧化工艺在处理各种废水(包含外部干扰,例如重金属)和/或不同处理方法方面的未来应用。策略(例如从侧流到主流)。75 mmol / L的Fe(II)。这项研究提供了有关厌氧氨氧化细菌对外部影响(增强和抑制)的生理反应的更多信息,并且还可能指导厌氧氨氧化工艺在处理各种废水(包含外部干扰,例如重金属)和/或不同处理方法方面的未来应用。策略(例如从侧流到主流)。

更新日期:2020-08-06
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