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Insights into Ethiopian honey bee diversity based on wing geomorphometric and mitochondrial DNA analyses
Apidologie ( IF 2.4 ) Pub Date : 2020-08-06 , DOI: 10.1007/s13592-020-00796-9
Teweldemedhn Gebretinsae Hailu , Paul D’Alvise , Adam Tofilski , Stefan Fuchs , Juergen Greiling , Peter Rosenkranz , Martin Hasselmann

Traditional beekeeping has been playing important socio-economic roles in Ethiopia for millennia. The country is situated in northeast Africa, where ranges of major evolutionary lineages of Apis mellifera adjoin. However, studies on the classification and distribution of subspecies and lineages of honey bees in the country are partly inconsistent, either proposing multiple subspecies and lineages or a unique A. m. simensis. This study was conducted with the aim of elucidating Ethiopian honey bees in reference to African subspecies and major global lineages using wing geometric morphometrics and COI-COII mitochondrial DNA analyses. For this purpose, 660 worker bees were collected from 66 colonies representing highland, midland, and lowland zones in different locations. Both methods indicated that the samples from this study form a distinct cluster together with A. m. simensis reference. In addition, forewing venation patterns showed that most of the Ethiopian samples are separate from all reference subspecies, except A. m. simensis. Analysis of COI-COII sequences revealed five DraI haplotypes (Y2, Y1, A1, and O5’), of which one was new denoted as Y3. Moreover, centroid size strongly associated with elevation. In conclusion, the results supported that Ethiopian honey bees are distinct both at lineage and subspecies levels; however, there is an indication of lineage O in the north.

中文翻译:

基于翅膀地貌测量和线粒体 DNA 分析对埃塞俄比亚蜜蜂多样性的洞察

几千年来,传统养蜂业一直在埃塞俄比亚发挥着重要的社会经济作用。该国位于非洲东北部,蜜蜂的主要进化谱系范围毗邻。然而,国内对蜜蜂亚种和谱系的分类和分布的研究部分不一致,要么提出了多个亚种和谱系,要么提出了独特的A.m. simensis。进行这项研究的目的是使用翅膀几何形态计量学和 COI-COII 线粒体 DNA 分析阐明埃塞俄比亚蜜蜂与非洲亚种和主要全球谱系的关系。为此,从代表不同地点的高地、中部和低地地区的 66 个蜂群中收集了 660 只工蜂。两种方法都表明,来自本研究的样本与 A. m. 形成了一个独特的集群。simensis 参考。此外,前翅脉络模式表明,大多数埃塞俄比亚样本与所有参考亚种分开,除了 A. m. simensis。COI-COII 序列的分析揭示了五种 DraI 单倍型(Y2、Y1、A1 和 O5'),其中一个是新的,表示为 Y3。此外,质心大小与海拔高度相关。总之,结果支持埃塞俄比亚蜜蜂在谱系和亚种水平上都是不同的;然而,在北方有血统 O 的迹象。COI-COII 序列的分析揭示了五种 DraI 单倍型(Y2、Y1、A1 和 O5'),其中一个是新的,表示为 Y3。此外,质心大小与海拔高度相关。总之,结果支持埃塞俄比亚蜜蜂在谱系和亚种水平上都是不同的;然而,在北方有血统 O 的迹象。COI-COII 序列的分析揭示了五种 DraI 单倍型(Y2、Y1、A1 和 O5'),其中一个是新的,表示为 Y3。此外,质心大小与海拔高度相关。总之,结果支持埃塞俄比亚蜜蜂在谱系和亚种水平上都是不同的;然而,在北方有血统 O 的迹象。
更新日期:2020-08-06
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