Purpose

Cysticercosis is the presence of Taenia solium larvae in humans or swines tissues. It is a public health problem related to bad hygienic habits and consumption of infected pork. T. crassiceps is a widely used cysticercosis experimental model. The combination of two effective drugs such as nitazoxanide (NTZ) and flubendazole (FBZ) may potentialize their effect. The aim of this study was to use biochemical analysis to determine the metabolic impact of the combination of NTZ and FBZ on cysticerci inoculated intraperitoneally in mice.

Methods

Balb/c mice intraperitoneally infected with T. crassiceps cysticerci received a single oral dose NTZ/FBZ (50 mg/kg). 24 h after the treatment the cysticerci were removed, frozen and analyzed by high performance liquid chromatography regarding the detection of the following metabolic pathways: glycolysis, gluconeogenesis, homolactic fermentation, tricarboxylic acid cycle, proteins catabolism and fatty acids oxidation.

Results

The treatment with the drugs combination induced a statistically significant increase in gluconeogenesis and in protein catabolism when compared to the control groups.

Conclusion

The drugs combination is potentialized and capable of causing greater metabolic stress than the separate treatment with NTZ or FBZ, showing its potential for an alternative cysticercosis treatment.

中文翻译：

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硝唑尼特和氟苯达唑的组合在体内治疗诱导粗绦虫囊尾蚴糖异生和蛋白质分解代谢。

目的

囊尾蚴病是人类或猪组织中存在猪带绦虫幼虫。这是一个与不良卫生习惯和食用受感染猪肉有关的公共卫生问题。T. crassiceps是一种广泛使用的囊尾蚴病实验模型。硝唑尼特 (NTZ) 和氟苯达唑 (FBZ) 等两种有效药物的组合可能会发挥其作用。本研究的目的是使用生化分析来确定 NTZ 和 FBZ 的组合对小鼠腹膜内接种的囊尾蚴的代谢影响。

方法

腹膜内感染粗尾蚴的 Balb/c 小鼠接受单次口服剂量的 NTZ/FBZ (50 mg/kg)。处理后 24 小时，囊尾蚴被取出、冷冻并通过高效液相色谱分析以下代谢途径的检测：糖酵解、糖异生、同型乳酸发酵、三羧酸循环、蛋白质分解代谢和脂肪酸氧化。

结果

与对照组相比，用药物组合治疗引起糖异生和蛋白质分解代谢的统计学显着增加。

结论

与单独使用 NTZ 或 FBZ 治疗相比，该药物组合具有潜力并且能够引起更大的代谢压力，显示出其作为替代囊尾蚴病治疗的潜力。