当前位置: X-MOL 学术Food Funct. › 论文详情
Our official English website, www.x-mol.net, welcomes your feedback! (Note: you will need to create a separate account there.)
ACE inhibitory peptides derived from de-fatted lemon basil seeds: optimization, purification, identification, structure-activity relationship and molecular docking analysis.
Food & Function ( IF 6.1 ) Pub Date : 2020-08-05 , DOI: 10.1039/d0fo01240h
Norhameemee Kheeree 1 , Papassara Sangtanoo 2 , Piroonporn Srimongkol 2 , Tanatorn Saisavoey 2 , Onrapak Reamtong 3 , Kiattawee Choowongkomon 4 , Aphichart Karnchanatat 2
Affiliation  

The oil processing industry generates significant quantities of lemon basil seed residue which is not currently used to any significant extent. However, this by-product has important potential as a source of bioactive peptides which may play a role as ingredients in functional foods. This study therefore sought to optimize the preparation techniques used to obtain the necessary protein hydrolysate from de-fatted lemon basil seeds (DLBS), and subsequently to examine the ACE inhibitory capabilities of the resulting hydrolysate. Response Surface Methodology (RSM) was used for the hydrolysis of DLBS by Alcalase®, with observation of the resulting ACE inhibitory activity and degree of hydrolysis (DH). The optimum conditions were 55 °C and 103 minutes with a ratio of enzyme to substrate of 7.0% w/v. The hydrolysate was fractionated by ultrafiltration and purified through RP-HPLC. The results reveal that the F2 sub-fraction demonstrated the highest ACE inhibitory activity. The amino acid sequence of this peak was identified by mass spectrometry as LGRNLPPI and GPAGPAGL with a molecular weight of 879.06 and 639.347 Dalton, respectively. These peptides were classified as non-toxic and bitter peptides. For the synthesized version of these peptides, the ACE inhibitory activity values, measured by IC50, were 0.124 ± 0.02 mM and 0.013 ± 0.001 mM, respectively. Analysis of the Lineweaver–Burk plot confirmed that these peptides served as non-competitive ones. The study of molecular docking showed that the ACE inhibitory behavior of both purified peptides was mainly due to the interactions of the hydrogen bonds between the peptides and ACE. It is therefore suggested that DLBS may be a useful raw material allowing the production of antihypertensive peptides which can offer therapeutic and commercial benefits as an ingredient in functional foods.

中文翻译:

脱脂柠檬罗勒种子中的ACE抑制肽:优化,纯化,鉴定,构效关系和分子对接分析。

石油加工行业会产生大量柠檬罗勒种子残留物,目前尚无大量使用。但是,这种副产物作为生物活性肽的来源具有重要的潜力,可以作为功能食品的成分发挥作用。因此,本研究寻求优化用于从脱脂柠檬罗勒种子(DLBS)中获得必要的蛋白质水解产物的制备技术,并随后检查所得水解产物的ACE抑制能力。响应表面方法学(RSM)用于通过Alcalase®水解DLBS,观察所得的ACE抑制活性和水解度(DH)。最佳条件是55°C和103分钟,酶与底物的比例为7.0%w / v。通过超滤将水解产物分级,并通过RP-HPLC纯化。结果表明,F2个子级分显示出最高的ACE抑制活性。通过质谱法将该峰的氨基酸序列鉴定为分子量分别为879.06和639.347道尔顿的LGRNLPPI和GPAGPAGL。这些肽被分类为无毒和苦涩的肽。对于这些肽的合成形式,通过IC 50测量的ACE抑制活性值分别为0.124±0.02 mM和0.013±0.001 mM。对Lineweaver-Burk图的分析证实了这些肽是非竞争性肽。分子对接研究表明,两种纯化肽的ACE抑制行为主要归因于肽与ACE之间的氢键相互作用。因此,建议DLBS可能是有用的原料,其允许生产抗高血压肽,作为功能食品中的成分可以提供治疗和商业利益。
更新日期:2020-09-23
down
wechat
bug