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SARS-CoV-2 genome analysis of strains in Pakistan reveals GH, S and L clade strains at the start of the pandemic
bioRxiv - Genomics Pub Date : 2020-08-04 , DOI: 10.1101/2020.08.04.234153 Najia Karim Ghanchi , Kiran Iqbal Masood , Asghar Nasir , Waqasuddin Khan , Syed Hani Abidi , Saba Shahid , Syed Faisal Mahmood , Akbar Kanji , Safina Razzak , Zeeshan Ansar , Nazneen Islam , M. B. Dharejo , Zahra Hasan , Rumina Hasan
bioRxiv - Genomics Pub Date : 2020-08-04 , DOI: 10.1101/2020.08.04.234153 Najia Karim Ghanchi , Kiran Iqbal Masood , Asghar Nasir , Waqasuddin Khan , Syed Hani Abidi , Saba Shahid , Syed Faisal Mahmood , Akbar Kanji , Safina Razzak , Zeeshan Ansar , Nazneen Islam , M. B. Dharejo , Zahra Hasan , Rumina Hasan
Objectives: Pakistan has a high infectious disease burden with about 265,000 reported cases of COVID-19. We investigated the genomic diversity of SARS-CoV-2 strains and present the first data on viruses circulating in the country.
Methods: We performed whole-genome sequencing and data analysis of SARS-CoV-2 eleven strains isolated in March and May. Results: Strains from travelers clustered with those from China, Saudi Arabia, India, USA and Australia. Five of eight SARS-CoV-2 strains were GH clade with Spike glycoprotein D614G, Ns3 gene Q57H, and RNA dependent RNA polymerase (RdRp) P4715L mutations. Two were S (ORF8 L84S and N S202N) and three were L clade and one was an I clade strain. One GH and one L strain each displayed Orf1ab L3606F indicating further evolutionary transitions. Conclusions: This data reveals SARS-CoV-2 strains of L, G, S and I have been circulating in Pakistan from March, at the start of the pandemic. It indicates viral diversity regarding infection in this populous region. Continuing molecular genomic surveillance of SARS-CoV-2 in the context of disease severity will be important to understand virus transmission patterns and host related determinants of COVID-19 in Pakistan.
更新日期:2020-08-05