Environmental DNA (eDNA) analysis is a method of detecting DNA from environmental samples and is used as a biomonitoring tool. In recent studies, Illumina MiSeq has been the most extensively used tool for eDNA metabarcoding. The Illumina iSeq 100 (hereafter, iSeq), one of the high-throughput sequencers (HTS), has a relatively simple workflow and is potentially more affordable than other HTS. However, its utility in eDNA metabarcoding has still not been investigated. In the present study, we applied fish eDNA metabarcoding to 40 water samples from river and lake ecosystems to assess the difference in species detectability and composition between iSeq and MiSeq. To check differences in sequence quality and errors, we also assessed differences in read changes between the two HTS. There were similar sequence qualities between iSeq and MiSeq. Significant difference was observed in the number of species between two HTS, but no difference was observed in species composition between the two HTS. Additionally, the species compositions in common with the conventional method were the same between the two HTS. According to the results, using the same amplicon library for sequencing, two HTS would exhibit a similar performance of fish species detection using eDNA metabarcoding.

中文翻译：

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Illumina iSeq 100 和 MiSeq 在淡水鱼环境 DNA 元条形码中表现出相似的性能

环境 DNA (eDNA) 分析是一种从环境样本中检测 DNA 的方法，用作生物监测工具。在最近的研究中，Illumina MiSeq 已成为最广泛使用的 eDNA 元条形码工具。Illumina iSeq 100（以下简称 iSeq）是一种高通量测序仪 (HTS)，其工作流程相对简单，并且可能比其他 HTS 更实惠。然而，它在 eDNA 元条形码中的效用仍未得到研究。在本研究中，我们将鱼类 eDNA 元条形码应用于来自河流和湖泊生态系统的 40 个水样，以评估 iSeq 和 MiSeq 之间物种可检测性和组成的差异。为了检查序列质量和错误的差异，我们还评估了两个 HTS 之间读取变化的差异。iSeq 和 MiSeq 之间的序列质量相似。两个HTS之间的物种数量存在显着差异，但两个HTS之间的物种组成没有差异。此外，两种HTS之间的物种组成与常规方法相同。根据结果​​，使用相同的扩增子库进行测序，两个 HTS 将表现出使用 eDNA 元条形码进行鱼类物种检测的相似性能。