The mobility of proteins and lipids within the cell, sculpted often times by the organisation of the membrane, reveals a great wealth of information on the function and interaction of these molecules as well as the membrane itself. Single particle tracking has proven to be a vital tool to study the mobility of individual molecules and unravel details of their behaviour. Interferometric scattering (iSCAT) microscopy is an emerging technique well suited for visualising the diffusion of gold nanoparticle-labelled membrane proteins to a spatial and temporal resolution beyond the means of traditional fluorescent labels. We discuss the applicability of interferometric single particle tracking (iSPT) microscopy to investigate the minutia in the motion of a protein through measurements visualising the mobility of the epidermal growth factor receptor in various biological scenarios on the live cell.

中文翻译：

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高精度蛋白质跟踪和干涉散射显微镜

细胞内蛋白质和脂质的移动性通常是通过膜的组织来雕刻的，揭示了关于这些分子以及膜本身的功能和相互作用的大量信息。事实证明，单粒子跟踪是研究单个分子的活动性和揭示其行为细节的重要工具。干涉散射（iSCAT）显微镜技术是一种新兴技术，非常适合可视化金纳米颗粒标记的膜蛋白在传统荧光标记方法无法实现的时空分辨率下的扩散。