Obesity and its related diseases have been associated with oxidative stress. Thus, the search for nutritional strategies to ameliorate oxidative stress in obese individuals seems important. We hypothesized that the supplementation with monounsaturated (2-hydroxyoleic acid (2-OHOA)) and with combined n-3 polyunsaturated (eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA)) fatty acids would ameliorate oxidative stress in different organs, including brain, liver, lungs, and kidneys of adult diet-induced obese (DIO) mice. Adult female ICR-CD1 mice were fed a high-fat diet (HFD) for 14 weeks. During the last 6 weeks of HFD feeding, one group of DIO mice received the same HFD, supplemented with 1500 mg of 2-OHOA per kg of HFD and another group with 1500 mg of EPA and 1500 mg of DHA per kg of HFD. At the end of the experiment, several parameters of oxidative stress were assessed. The supplementation with 2-OHOA or with EPA and DHA in DIO mice was able to revert oxidative stress, enhancing the activities of catalase and glutathione reductase, as well as diminishing the activity of xanthine oxidase, the concentration of thiobarbituric acid reactive substances (TBARS) and the ratio between oxidized glutathione and reduced glutathione in several organs. These reached similar values to those of control mice, which were fed a standard diet. These data suggest that supplementation with 2-OHOA and with EPA and DHA could be an effective nutritional intervention to restore an appropriate redox state in DIO mice.

肥胖及其相关疾病已与氧化应激相关。因此，寻找减轻肥胖者氧化应激的营养策略似乎很重要。我们假设补充单不饱和脂肪酸（2-羟基油酸（2-OHOA））和n-3多不饱和脂肪酸（二十碳五烯酸（EPA）和二十二碳六烯酸（DHA））组合脂肪酸将减轻包括大脑在内的不同器官的氧化应激饮食诱发的肥胖（DIO）小鼠的肝脏，肝脏，肺脏和肾脏。给成年雌性ICR-CD1小鼠喂养高脂饮食（HFD），持续14周。在HFD喂养的最后6周内，一组DIO小鼠接受相同的HFD，每千克HFD补充1500 mg 2-OHOA，另一组每千克HFD补充1500 mg EPA和1500 mg DHA。在实验结束时，评估了氧化应激的几个参数。在DIO小鼠中补充2-OHOA或EPA和DHA能够逆转氧化应激，增强过氧化氢酶和谷胱甘肽还原酶的活性，并减少黄嘌呤氧化酶的活性，硫代巴比妥酸反应性物质（TBARS）的浓度以及几个器官中氧化型谷胱甘肽和还原型谷胱甘肽的比例。这些达到与喂养标准饮食的对照小鼠相似的值。这些数据表明，补充2-OHOA以及EPA和DHA可能是恢复DIO小鼠适当氧化还原状态的有效营养干预措施。增强过氧化氢酶和谷胱甘肽还原酶的活性，并降低黄嘌呤氧化酶的活性，硫代巴比妥酸反应性物质（TBARS）的浓度以及几种器官中氧化型谷胱甘肽与还原型谷胱甘肽的比例。这些达到与喂养标准饮食的对照小鼠相似的值。这些数据表明，补充2-OHOA以及EPA和DHA可能是恢复DIO小鼠适当氧化还原状态的有效营养干预措施。增强过氧化氢酶和谷胱甘肽还原酶的活性，并降低黄嘌呤氧化酶的活性，硫代巴比妥酸反应性物质（TBARS）的浓度以及几种器官中氧化型谷胱甘肽与还原型谷胱甘肽的比例。这些达到与喂养标准饮食的对照小鼠相似的值。这些数据表明，补充2-OHOA以及EPA和DHA可能是恢复DIO小鼠适当氧化还原状态的有效营养干预措施。