A novel and green procedure consisting of high hydrostatic pressure (HHP) aided by a commercial cellulase (Celluclast^®) has been applied to valorise the apple by-product, a valuable source of dietary fibre but mainly composed by insoluble fibre. Optimal conditions for solubilisation of dietary fibre were first determined at atmospheric pressure as 2% (w/v) of substrate concentration and 20 Endo-Glucanase Units of cellulase. Monitoring of polysaccharides and oligosaccharides released from apple by-product was carried out by means of a newly validated HPLC method with refractive index detector. A synergistic effect was observed when the combined HHP plus cellulase treatment was used. Thus, the application of 200 MPa at 50 °C for 15 min enabled a significant increase in the release of water-soluble polysaccharides (1.8-fold) and oligosaccharides (3.8-fold), as well as a considerable decrease in the time required (up to 120-fold), compared to control at 0.1 MPa. Therefore, this technology could be a promising alternative approach to transform an industrial by-product into a novel rich-in-oligosaccharide food ingredient and a step forward into shaping the world of prebiotics.

中文翻译：

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Celluclast®辅助的高静水压力会从Apple副产品中释放寡糖。

由通过商业纤维素酶（计算机辅助的Celluclast高静水压（HHP）的一种新颖的和绿色的过程^®）已经施加到valorise副产品苹果，膳食纤维的宝贵来源但主要由不溶性纤维组成。首先在大气压下确定膳食纤维增溶的最佳条件为2％（w / v）底物浓度和20内切葡聚糖酶纤维素酶。苹果副产物中释放出的多糖和低聚糖的监测是通过新近验证的带折光检测器的HPLC方法进行的。当联合使用HHP和纤维素酶处理时，观察到协同作用。因此，在50°C下施加200 MPa的压力15分钟可以显着增加水溶性多糖（1.8倍）和低聚糖（3.8倍）的释放，并显着减少所需时间（最高为120倍），而对照组为0.1 MPa。因此，这项技术可能是一种有前途的替代方法，可以将工业副产品转化为一种新型的富含低聚糖的食品成分，并且可以迈向塑造益生元世界的一步。