Antimicrobial resistance among isolates from wild animals is increasingly reported. Extended‐spectrum beta‐lactamase (ESBL)‐producing Enterobacteriaceae, and particularly Escherichia coli, have spread worldwide as one of the most common multidrug‐resistant organisms. The aim of this study was to determine the carriage rate of ESBL‐producing E. coli isolates and their genetic characteristics in wild vultures from the Canary Islands. Faecal samples were collected from 22 apparently healthy free‐ranging (wild) vulture chicks from Lanzarote and Fuerteventura (Canary Islands) during July 2019. They were seeded in MacConkey agar supplemented with cefotaxime (2 μg ml⁻¹). Colonies with typical morphology of E. coli were identified by MALDI‐TOF‐MS. Antimicrobial susceptibility was done by disk diffusion. Phenotypic detection of ESBL was performed by double‐disk tests. The presence of bla_CTX‐M, bla_SHV, bla_TEM, bla_KPC and bla_OXA‐48 genes, as well as mcr‐1 (colistin resistance), tetA/tetB and int1 gene, was tested by PCR/sequencing. Phylogenetic groups and multilocus sequence typing (MLST) were determined by PCR/sequencing. ESBL‐producing E. coli isolates were detected in 5/22 tested animals (22.7%), and all isolates (one/animal) carried bla_CTX‐M genes: bla_CTX‐M‐15 (n = 3) and bla_CTX‐M‐55 (n = 2). ESBL‐positive isolates were ascribed to phylogenetic group D (two isolates), B₁ (two isolates) and A (one isolate), and five sequence types were detected (ST/phylogenetic‐group/ESBL): ST515/B1/CTX‐M‐15, ST1290/A/CTX‐M‐15, ST38/D/CTX‐M‐15, ST457/D/CTX‐M‐55 and ST6448/B₁/CTX‐M‐55; this suggests a genetic diversity among these isolates. Three CTX‐M‐15‐producing isolates contained the bla_TEM gene and one the tetA gene. To our knowledge, this appears to be the first report of ESBL‐producing E. coli in vulture chicks from the Canary Islands.

中文翻译：

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加那利群岛秃v中产生广谱β-内酰胺酶的大肠杆菌的分子多样性。

越来越多地报道了来自野生动物的分离株之间的抗药性。产生超广谱β-内酰胺酶（ESBL）的肠杆菌科细菌，尤其是大肠杆菌，已作为最常见的多药耐药生物之一在世界范围内传播。这项研究的目的是确定产生ESBL的E的携带率。加那利群岛野生秃鹰中的大肠杆菌分离株及其遗传特征。粪便样品于2019年7月从Lanzarote和Fuerteventura（加那利群岛）的22只看似健康的自由放养（野生）雏鸡中收集。它们接种在补充有头孢噻肟（2μgml ^-1）的MacConkey琼脂中。具有典型形态的菌落Ë。MALDI-TOF-MS鉴定了大肠杆菌。细菌敏感性通过盘扩散来完成。通过双盘测试对ESBL进行表型检测。的存在BLA _CTX-M ，BLA _SHV，BLA _TEM，BLA _KPC和BLA _OXA-48基因，以及MCR- 1（粘菌素抗性），TET A / TET B和INT 1基因，通过PCR检测/测序。系统发育组和多基因座序列分型（MLST）通过PCR /测序确定。产ESBL ê。大肠杆菌在5/22只经测试的动物中检出了分离株（22.7％），所有分离株（一只/动物）都带有bla _CTX-M基因：bla _CTX-M-15（n = 3）和bla _CTX-M-55（n = 2）。ESBL阳性分离株归因于系统发生组D（两个分离株），B ₁（两个分离株）和A（一个分离株），并检测到五种序列类型（ST /系统发生组/ ESBL）：ST515 / B1 / CTX- M‐15，ST1290 / A / CTX‐M‐15，ST38 / D / CTX‐M‐15，ST457 / D / CTX‐M‐55和ST6448 / B ₁ / CTX‐M‐55; 这表明这些分离株之间存在遗传多样性。三种产生CTX‐M‐15的分离株包含bla _TEM基因，另一种则是tet一个基因。据我们所知，这似乎是ESBL生产E的第一份报告。来自加那利群岛的秃鹰雏鸡中的大肠杆菌。