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Escherichia coli survival in plasma‐treated water and in a gas–liquid plasma reactor
Plasma Processes and Polymers ( IF 3.5 ) Pub Date : 2020-08-05 , DOI: 10.1002/ppap.202000099
Cesar Rodriguez 1 , Robert J. Wandell 2 , Zhiming Zhang 3 , Julie M. Neurohr 4 , Youneng Tang 3 , Ryan Rhodes 4 , Stephen T. Kinsey 4 , Bruce R. Locke 2
Affiliation  

The effects of post‐plasma exposure to deionized water treated by argon–water plasma and to direct plasma treatment of bacteria in a liquid solution of a strain of Escherichia coli, which was independently evolved to resist the bactericidal effects of hydrogen peroxide, were determined. The hydrogen peroxide resistant phenotype of the evolved strain was proportional to the initial cell density. The catalase activity was significantly elevated in the evolved strain as compared with the wild‐type strain, and the evolved strain resisted the bactericidal effects of plasma‐treated water (post‐plasma exposure) as compared with the wild‐type strain. The evolved strain remained viable after flowing through the plasma reactor and lysed less when exposed to (nonplasma control) hydrogen peroxide solutions as compared with the wild‐type cells.

中文翻译:

大肠杆菌在血浆处理过的水和气液血浆反应器中的存活率

等离子暴露于氩水等离子体处理过的去离子水中以及直接对大肠杆菌菌株液体溶液中的细菌进行等离子体处理的影响确定了其独立进化以抵抗过氧化氢的杀菌作用的方法。进化菌株的抗过氧化氢表型与初始细胞密度成正比。与野生型菌株相比,进化菌株中的过氧化氢酶活性显着提高,并且进化菌株抵抗血浆处理水的杀菌作用(血浆暴露后)。与野生型细胞相比,进化后的菌株在流过血浆反应器后仍保持活力,并且暴露于(非血浆对照)过氧化氢溶液时裂解较少。
更新日期:2020-08-05
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