Grape dehydration is an oenological process used for production of high‐quality reinforced and sweet wines. High‐resolution mass spectrometry (ultrahigh‐performance liquid chromatography/quadrupole time‐of‐flight [UHPLC/QTOF]) was used to deepen the characterization of some flavonoids previously proposed in Corvina and Raboso Piave withered grapes. By performing a targeted data analysis workflow and orthogonal identification approaches (tandem mass spectrometry [MS/MS]), in silico fragmentation, and calculation of putative retention time), elucidation on the structures of six compounds previously proposed was achieved (taxifolin‐pentoside, two tetrahydroxyflavanone‐hexoside derivatives, a tetrahydroxy‐dimethoxyflavanone‐hexoside derivative, a pentahydroxyflavone, and peonidin‐O‐pentoside); and the structures of four putative new grape flavonoids were characterized (dihydromyricetin‐O‐hexoside, taxifolin‐di‐O‐hexoside, isorhamnetin, and a pinoquercetin isomer). Findings enlarge the panorama of flavonoids in grape and of their possible biosynthetic pathways.

中文翻译：

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通过四极杆飞行时间质谱法在采后枯萎的葡萄 (Vitis vinifera L.) 中鉴定出一些推定的黄酮类化合物的结构。

葡萄脱水是一种用于生产高品质加强型和甜型葡萄酒的酿酒工艺。高分辨率质谱法（超高效液相色谱/四极杆飞行时间 [UHPLC/QTOF]）用于加深先前在 Corvina 和 Raboso Piave 枯萎葡萄中提出的一些黄酮类化合物的表征。通过执行靶向数据分析工作流程和正交鉴定方法（串联质谱 [MS/MS]）、计算机碎裂和推定保留时间的计算），实现了对先前提出的六种化合物（紫杉叶素-戊糖苷、两种四羟基黄烷酮-己糖苷衍生物、一种四羟基-二甲氧基黄烷酮-己糖苷衍生物、一种五羟基黄酮和芍药苷-O-戊糖苷）；并表征了四种假定的新葡萄黄酮类化合物（二氢杨梅素-O-己糖苷、紫杉叶素-二-O-己糖苷、异鼠李素和松香素异构体）的结构。研究结果扩大了葡萄中黄酮类化合物及其可能的生物合成途径的全景图。