Food allergy is related to increasing risk of the development of allergic asthma, but the precise interplay between sensitization to different allergens in different compartments of the body is not fully understood. The aim of this study was to develop a novel humanized murine model of mixed food and respiratory allergy that recapitulates the human anaphylactic response and to more clearly understand the impact of food allergies on asthma. Immunodeficient mice transferred with peripheral blood mononuclear cells (PBMCs) from donors with peanut and house dust mite (HDM) allergy were exposed and challenged to peanut. Between peanut exposure and challenge, mice were intranasally treated to HDM. Allergic parameters were analyzed. Allergen‐specific immunoglobulin (Ig)E in sera could only be measured in mice treated with peripheral blood mononuclear cells (PBMCs) plus allergen. A preceding peanut exposure increased IgE levels, histamine release, bronchial hyper‐responsiveness and lung inflammation. Recruitment of inflammatory cells to the airways was aggravated associated with an enhanced T helper type 2 (Th2)/Th17 cytokine secretion when the two allergies were present. A preceding peanut exposure amplifies allergic asthma in this humanized model, which may contribute to the understanding of underlying immunological mechanism of polysensitization occurring in allergic individuals and evaluation of therapeutic interventions.

中文翻译：

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食物过敏会促进 Th2/Th17 反应，从而在人源化小鼠中驱动屋尘螨引起的过敏性气道炎症。

食物过敏与过敏性哮喘发生风险的增加有关，但人体不同部位对不同过敏原的敏感性之间的确切相互作用尚不完全清楚。本研究的目的是开发一种新的混合食物和呼吸道过敏的人源化小鼠模型，以概括人类过敏反应，并更清楚地了解食物过敏对哮喘的影响。从花生和屋尘螨 (HDM) 过敏的供体移植外周血单核细胞 (PBMC) 的免疫缺陷小鼠暴露并受到花生的挑战。在花生暴露和攻击之间，对小鼠进行鼻内处理以接受 HDM。分析了过敏参数。血清中的过敏原特异性免疫球蛋白 (Ig)E 只能在用外周血单核细胞 (PBMC) 加过敏原处理的小鼠中测量。之前的花生暴露会增加 IgE 水平、组胺释放、支气管高反应性和肺部炎症。当存在两种过敏症时，炎症细胞向气道的募集与增强的 2 型 T 辅助细胞 (Th2)/Th17 细胞因子分泌有关。在此人性化模型中，先前的花生暴露会放大过敏性哮喘，这可能有助于了解过敏个体中发生的多敏化的潜在免疫机制和治疗干预措施的评估。当存在两种过敏症时，炎症细胞向气道的募集与增强的 2 型 T 辅助细胞 (Th2)/Th17 细胞因子分泌有关。在此人性化模型中，先前的花生暴露会放大过敏性哮喘，这可能有助于了解过敏个体中发生的多敏化的潜在免疫机制和治疗干预措施的评估。当存在两种过敏症时，炎症细胞向气道的募集与增强的 2 型 T 辅助细胞 (Th2)/Th17 细胞因子分泌有关。在此人性化模型中，先前的花生暴露会放大过敏性哮喘，这可能有助于了解过敏个体中发生的多敏化的潜在免疫机制和治疗干预措施的评估。