Detonation‐produced nanodiamond, both as a powder (with adsorbed water) and especially when suspended in an aqueous colloid, can support the growth (both aerobic and anaerobic) of bacteria and fungi. These were isolated and identified by microbiological methods, optical and electron microscopy, as species of Penicillium, Purpureocillium, Beaveria, Trichoderma and Aspergillus genera. The C : N molar ratio of the developing fibers (comprising fungal mycelia with attached bacteria and entrapped nanodiamond) decreased from 25 to 11 between the 1^st and 10^th week of incubation (cf. 40 in initial nanodiamond, 4.6 typical for bacteria and 8.3 for fungi), and from 4 to <1 after the 12^th week, as the lysis of microorganisms releases carbon as CO₂ and nitrogen as NH₄⁺ or NO₃⁻. The nitrogen content of the colloid increased by an order of magnitude and more, due to fixation of N₂ by nanodiamond under ambient conditions.The process requires water but not necessarily oxygen present.

中文翻译：

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纳米金刚石胶体溶液的固氮和生物行为。

爆轰产生的纳米金刚石，既是粉末状（带有吸附水），尤其是悬浮在水性胶体中时，可以支持细菌和真菌的生长（需氧和厌氧）。这些被分离并通过微生物学方法，光学和电子显微镜鉴定为青霉菌，紫癜菌，海狸属，木霉属和曲霉属。在培养的^第1周到^第10周之间，发育中的纤维（包含附着有细菌的真菌菌丝体和纳米金刚石）的C：N摩尔比从25降低至11 （参见初始纳米金刚石中的40，细菌典型值4.6和8.3） （对于真菌），并且在第12^天后从4降至<1周，作为微生物释放碳的溶解的CO ₂和氮作为NH ₄ ⁺或NO ₃ ^- 。由于在环境条件下纳米金刚石将N ₂固定，胶体中的氮含量增加了一个数量级甚至更多。该过程需要水，但不一定要存在氧气。