Escherichia coli is one of the most diverse microbial species. Pathogenic E. coli is capable of causing various diseases in humans, including several types of diarrhea, urinary tract infections, sepsis, and meningitis. This study focused on the antibiotic susceptibility profile and genomic analysis of a clinical E. coli Guangzhou-Eco330 isolated from a hospitalized 8-year-old female patient suffered from pulmonary infection in 2017. Susceptibility to 15 antibiotics were determined using Vitek2™ Automated Susceptibility System and Etest strips and interpreted based on CLSI guidelines. The genome was sequenced using Illumina Hiseq 2500 platform and assembled de novo using Velvet, followed by bioinformatics analysis. The genome has a length of 5,132,642 bp and contains 4989 predicted genes with an average GC content of 50.51%. The carriage of rfbE gene suggested the strain belonging to O157. In the genome, 70 non-coding RNAs, 50 repeat sequences, 18 transposons, 78 GIs, 9 CRISPRs, and 3 large prophages were identified. 37 PHI related genes and 108 virulence genes were determined to contribute to its pathogenicity. Specifically, the acquisition of multiple antibiotic resistance genes including bla_CTX-M-55, bla_OXA-10, bla_CMY-48, tetB, and qnrS1 contributed to its resistance to penicillins, telracyclines, cephalosporin, and quinolones. The understanding of the genome may aid in further study on the clinical control of multi-drug resistance E. coli.

大肠杆菌是最多样化的微生物之一。致病性大肠杆菌能够在人类中引起多种疾病，包括几种类型的腹泻，尿路感染，败血症和脑膜炎。该研究侧重于2017年从住院的8岁女性肺部感染患者中分离出的临床大肠杆菌Guangzhou-Eco330的抗生素敏感性概况和基因组分析。使用Vitek2™自动敏感性系统确定了对15种抗生素的敏感性和Etest条，并根据CLSI指南进行解释。使用Illumina Hiseq 2500平台对基因组进行测序并从头开始组装使用Velvet，然后进行生物信息学分析。基因组长度为5,132,642 bp，包含4989个预测基因，平均GC含量为50.51％。rfbE基因的携带表明该菌株属于O157。在基因组中，鉴定出70个非编码RNA，50个重复序列，18个转座子，78个GI，9个CRISPR和3个大噬菌体。确定了37个PHI相关基因和108个毒力基因有助于其致病性。具体而言，获得多个抗生素抗性基因，包括bla _CTX-M-55，bla _OXA-10，bla _CMY-48，tetB和qnrS1有助于抵抗青霉素，泰拉西林，头孢菌素和喹诺酮。对基因组的了解可能有助于进一步研究对多药耐药性大肠杆菌的临床控制。