Frequent exposure to varied home cage sizes alters pain sensitivity and some key inflammation-related biomarkers.,Journal of Neuroscience Methods

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Frequent exposure to varied home cage sizes alters pain sensitivity and some key inflammation-related biomarkers.
Journal of Neuroscience Methods ( IF 3 ) Pub Date : 2020-08-05 , DOI: 10.1016/j.jneumeth.2020.108890
Aboyeji Lukuman Oyewole ₁ , Kehinde Olumide Oyafemi ₂ , Kolade Samson Badmus ₂ , Janet Omotola Omoleye ₂ , Midrar Folahanmi Abubakar ₂ , Omolade Adeniyi-Raheem ₂ , Abdul-Hameed Amedu ₂ , Dolapo Latifah Lawal ₂ , Aishat Oluwakemi Ijiyode ₂ , Ateeqah Oreoluwa Yussuf ₂ , Solomon Sunday Ishola ₂ , Fatimo Ajoke Sulaimon ₃ , Abdulmusawwir O Alli-Oluwafuyi ₄ , Abdulrazaq Bidemi Nafiu ₅ , Olugbenga Akinola ₆ , Olayemi Joseph Olajide ₇ , Abdulbasit Amin ₈ , Wahab Imam Abdulmajeed ₈ , Olugbenga Samuel Michael ₉ , Oluwaseun Aremu Adeyanju ₁₀ , Gbowoloye Lanre Ogunjimi ₁₁

Affiliation

Department of Physiology, Faculty of Basic Medical Sciences, University of Ilorin, Ilorin, Nigeria; Biotranslational Research Group, University of Ilorin, Ilorin, Nigeria; Bioscience Research Hub, Ilorin, Nigeria.
Department of Physiology, Faculty of Basic Medical Sciences, University of Ilorin, Ilorin, Nigeria; Bioresearch Hub Laboratory, Ilorin, Nigeria.
Department of Anatomy, Faculty of Basic Medical Sciences, University of Ilorin, Ilorin, Nigeria.
Bioresearch Hub Laboratory, Ilorin, Nigeria; Department of Pharmacology and Therapeutics, Faculty of Basic Medical Sciences, University of Ilorin, Ilorin, Nigeria.
Biotranslational Research Group, University of Ilorin, Ilorin, Nigeria; Departments of Human Physiology, Federal University Dutse, Jigawa, Nigeria.
Biotranslational Research Group, University of Ilorin, Ilorin, Nigeria; Department of Pharmacology and Therapeutics, Faculty of Basic Medical Sciences, University of Ilorin, Ilorin, Nigeria; Malaria Research Laboratories, Institute for Advanced Medical Research and Training, College of Medicine, University of Ibadan, Ibadan, Nigeria.
Biotranslational Research Group, University of Ilorin, Ilorin, Nigeria; Department of Anatomy, Faculty of Basic Medical Sciences, University of Ilorin, Ilorin, Nigeria.
Department of Physiology, Faculty of Basic Medical Sciences, University of Ilorin, Ilorin, Nigeria; Biotranslational Research Group, University of Ilorin, Ilorin, Nigeria.
Bioscience Research Hub, Ilorin, Nigeria; Cardiometabolic Research Unit, Departments of Physiology, College of Health Sciences, Bowen University, Iwo, Nigeria.
Bioscience Research Hub, Ilorin, Nigeria; Cardiometabolic Research Unit, Departments of Physiology, Afe Babalola University Ado-Ekiti, Ekiti State, Nigeria.
Bioresearch Hub Laboratory, Ilorin, Nigeria.

Background

Nature and size of rodent cages vary from one laboratory or country to another. Little is however known about the physiological implications of exposure to diverse cage sizes in animal-based experiments.

Method

Here, two groups of male Swiss mice (Control group – Cage stationed, and Test group – Cage migrated) were used for this study. The cage-migrated mice were exposed daily to various cage sizes used across laboratories in Nigeria while the cage-stationed mice exposed daily to different but the same cage size and shape. At the end of the 30 days exposure, top-rated paradigms were used to profile changes in physiological behaviours, and this was followed by evaluation of histological and biochemical metrics.

Results

The study showed a significant (p < 0.05) decrease in blood glucose levels (at 60 and 120 min of oral glucose tolerance test) in the cage-migrated mice compared to cage-stationed mice. Strikingly, peripheral oxidative stress (plasma malondialdehyde) and pain sensitivity (formalin test, hot-and-cold plate test, and von Frey test) decreased significantly in cage-migrated mice compared to cage-stationed animals. Also, the pro-inflammation mediators (IL-6 and NF-κB) increased significantly in cage-migrated mice compared to cage-stationed mice. However, emotion-linked behaviours, neurotransmitters (serotonin, noradrenaline and GABA), brain and plasma electrolytes were not significantly difference in cage-migrated animals compared to cage-stationed mice.

Conclusion

Taken together, these results suggest that varied size cage-to-cage exposure of experimental mice could affect targeted behavioural and biomolecular parameters of pain and inflammation, thus diminishing research reproducibility, precipitating false negative/positive results and leading to poor translational outcomes.

中文翻译：

经常暴露于各种大小的笼子中会改变疼痛敏感性和一些关键的炎症相关生物标志物。

背景

啮齿动物笼的性质和大小因一个实验室或国家而异。然而，在基于动物的实验中，关于暴露于不同笼尺寸的生理影响知之甚少。

方法

在此，使用两组雄性瑞士小鼠（对照组-笼养，测试组-笼养）进行了这项研究。笼子迁移的小鼠每天要暴露于尼日利亚各个实验室使用的各种笼子大小，而笼养的小鼠每天要暴露于不同但相同的笼子大小和形状。在暴露的30天结束时，使用顶级范例描述生理行为的变化，然后评估组织学和生化指标。

结果

研究表明，与笼养小鼠相比，笼养小鼠的血糖水平明显降低（p <0.05）（在口服葡萄糖耐量试验60和120分钟时）。令人惊讶的是，与笼养动物相比，笼养迁移的小鼠的外周氧化应激（血浆丙二醛）和疼痛敏感性（福尔马林试验，冷热平板试验和冯·弗雷试验）明显降低。而且，与笼养小鼠相比，笼养小鼠的促炎介质（IL-6和NF-κB）显着增加。然而，与笼养的小鼠相比，笼养的动物与情绪相关的行为，神经递质（血清素，去甲肾上腺素和GABA），脑和血浆电解质没有显着差异。