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Mesorhizobium tamadayense MM3441: A novel methylotroph with a great potential in degrading N,N′-dimethylformamide
International Biodeterioration & Biodegradation ( IF 4.8 ) Pub Date : 2020-09-01 , DOI: 10.1016/j.ibiod.2020.105045
Kartik Dhar , Suresh R. Subashchandrabose , Kadiyala Venkateswarlu , Mallavarapu Megharaj

Abstract The widespread presence of the versatile solvent, N,N′-dimethylformamide (DMF), in industrial wastewaters, and the growing concern over its ecotoxicity necessitate the implementation of an effective remediation technology. Mesorhizobium tamadayense MM3441, isolated from a soil contaminated with polycyclic aromatic hydrocarbons, utilized higher concentrations of DMF as sole carbon and nitrogen source. To our knowledge, the strain MM3441 is the only member of the genus, Mesorhizobium, capable of degrading DMF. This strain, with methylotrophic mode of nutrition, also exhibited resistance to several organic solvents. Optimal growth of the strain was observed at pH 7.0 in the presence of 5000 mg L−1 DMF. The strain removed 95% of 5000 mg L−1 DMF within nine days of incubation. DMF utilization was accompanied by the accumulation of dimethylamine (DMA) and ammonia in the culture medium. Both the resting cell and dimethylformamidase (DMFase) assays provided the circumstantial evidence that DMF degradation by the strain MM3441 proceeds via the typical pathway yielding DMA. However, DMFase expression is not inducible with DMF, suggesting that the enzyme is constitutively expressed. Based on the DMF degradation ability and metabolic flexibility of the strain MM3441, the present study advocates its potential use in bioremediation of the environments contaminated with DMF.

中文翻译:

Mesorhizobium tamadayense MM3441:一种在降解 N,N'-二甲基甲酰胺方面具有巨大潜力的新型甲基营养菌

摘要 通用溶剂 N,N'-二甲基甲酰胺 (DMF) 在工业废水中的广泛存在,以及对其生态毒性的日益关注,需要实施有效的修复技术。从被多环芳烃污染的土壤中分离出来的 Mesorhizobium tamadayense MM3441 利用更高浓度的 DMF 作为唯一的碳和氮源。据我们所知,菌株 MM3441 是 Mesorhizobium 属中唯一能够降解 DMF 的成员。这种具有甲基营养模式的菌株也表现出对几种有机溶剂的抵抗力。在 5000 mg L-1 DMF 存在下,在 pH 7.0 下观察到菌株的最佳生长。该菌株在孵育 9 天内去除了 95% 的 5000 mg L-1 DMF。DMF 的利用伴随着培养基中二甲胺 (DMA) 和氨的积累。静息细胞和二甲基甲酰胺酶 (DMFase) 测定都提供了间接证据,表明菌株 MM3441 对 DMF 的降解是通过产生 DMA 的典型途径进行的。然而,DMFase 表达不能被 DMF 诱导,表明该酶是组成型表达的。基于菌株 MM3441 的 DMF 降解能力和代谢灵活性,本研究主张其在被 DMF 污染的环境的生物修复中的潜在用途。DMFase 表达不能被 DMF 诱导,表明该酶是组成型表达的。基于菌株 MM3441 的 DMF 降解能力和代谢灵活性,本研究主张其在被 DMF 污染的环境的生物修复中的潜在用途。DMFase 表达不能被 DMF 诱导,表明该酶是组成型表达的。基于菌株 MM3441 的 DMF 降解能力和代谢灵活性,本研究主张其在被 DMF 污染的环境的生物修复中的潜在用途。
更新日期:2020-09-01
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