Bioremediation has gained global prominence as an effective method for treating hydrocarbon-contaminated drill mud waste (HCDW). However, the problem of low nutrient content, bioavailability and microbial presence remain largely unresolved. In this study, the synergistic effects of compost, cow bile and bacterial culture on the degradation rate of HCDW was investigated. A homogenized HCDW sample (80 kg) obtained from 25 different drill mud tanks was divided into 20 portions (4 kg each) and each adjusted to 1.4% nitrogen content + 20 ml cow bile (i.e., basic treatment). Pure cultures of Brevibacterium casei (Bc) and Bacillus zhangzhouensi (Bz) and their mixture (BcBz) were subsequently added to 12 of the amended HCDW (basic) to undergo a 6-week incubation. A portion of the unamended HCDW (2 kg) was used as control. Initial pH, electrical conductivity and surface tension values of the HCDW were 8.83, 2.34 mS/cm and 36.5 mN/m, respectively. Corresponding values for total petroleum hydrocarbon (TPH), total nitrogen and total plate count bacteria were 165 g/kg, 0.04% and 4.4 × 10² cfu/ml. The treatments led to a substantial reduction in TPH (p < 0.05) while the control had no significant effect (p > 0.05). TPH reduction after the experimental period occurred in the order: basic + BcBz (99.7%) > basic + Bz (99.5%) > basic + Bc (99.2%) > basic (95.2%) > control (0.06%). Multiple regression analysis revealed significant effect of total plate count, pH, CN ratio and electrical conductivity (R² = 0.87, p = 0.05) on the degradation of TPH in the HCDW. The study demonstrates strong interactive effects of compost, cow bile and bacteria culture on the remediation of HCDW, which can be applied to boost the efficiency of the bioremediation technique.

生物修复已作为一种有效的方法来处理烃类污染的钻探泥浆废物（HCDW），在全球范围内倍受关注。但是，营养成分低，生物利用度和微生物存在的问题仍未解决。在这项研究中，研究了堆肥，牛胆汁和细菌培养对HCDW降解速率的协同作用。从25个不同的钻探泥浆罐获得的均质HCDW样品（80千克）被分成20份（每份4千克），每份都调整为1.4％氮含量+ 20毫升牛胆汁（即基本处理）。干酪短杆菌（Bc）和漳州芽孢杆菌的纯培养物随后将（Bz）及其混合物（BcBz）添加到12个修改后的HCDW（碱性）中，进行6周的孵育。一部分未经修饰的HCDW（2千克）用作对照。HCDW的初始pH，电导率和表面张力值分别为8.83、2.34 mS / cm和36.5 mN / m。总石油烃（TPH），总氮和总板数细菌的对应值为165 g / kg，0.04％和4.4×10 ² cfu /毫升。这些处理导致TPH显着降低（p <0.05），而对照组则无显着影响（p> 0.05）。实验期后TPH降低的顺序为：碱性+ BcBz（99.7％）>碱性+ Bz（99.5％）>碱性+ Bc（99.2％）>碱性（95.2％）>对照（0.06％）。多元回归分析显示，总板数，pH，CN比和电导率（R ²  = 0.87，p = 0.05）对HCDW中TPH的降解具有显着影响。该研究表明堆肥，牛胆汁和细菌培养物对HCDW的修复具有很强的交互作用，可用于提高生物修复技术的效率。