当前位置: X-MOL 学术Biochem. Biophys. Res. Commun. › 论文详情
Our official English website, www.x-mol.net, welcomes your feedback! (Note: you will need to create a separate account there.)
The effects of membrane potential and extracellular matrix composition on vascular differentiation of cardiac progenitor cells.
Biochemical and Biophysical Research Communications ( IF 3.1 ) Pub Date : 2020-08-04 , DOI: 10.1016/j.bbrc.2020.06.149
Mark C Daley 1 , Mattia Bonzanni 2 , Allison M MacKenzie 1 , David L Kaplan 3 , Lauren D Black 4
Affiliation  

Historically, the field of tissue engineering has been adept at modulating the chemical and physical microenvironment. This approach has yielded significant progress, but it is imperative to further integrate our understanding of other fundamental cell signaling paradigms into tissue engineering methods. Bioelectric signaling has been demonstrated to be a vital part of tissue development, regeneration, and function across organ systems and the extracellular matrix is known to alter the bioelectric properties of cells. Thus, there is a need to bolster our understanding of how matrix and bioelectric signals interact to drive cell phenotype. We examine how cardiac progenitor cell differentiation is altered by simultaneous changes in both resting membrane potential and extracellular matrix composition. Pediatric c-kit+ cardiac progenitor cells were differentiated on fetal or adult cardiac extracellular matrix while being treated with drugs that alter resting membrane potential. Smooth muscle gene expression was increased with depolarization and decreased with hyperpolarization while endothelial and cardiac expression were unchanged. Early smooth muscle protein expression is modified by matrix developmental age, with fetal ECM appearing to amplify the effects of resting membrane potential. Thus, combining matrix composition and bioelectric signaling represents a potential alternative for guiding cell behavior in tissue engineering and regenerative medicine.



中文翻译:

膜电位和细胞外基质组成对心脏祖细胞血管分化的影响。

从历史上看,组织工程领域一直擅长于调节化学和物理微环境。这种方法已经取得了重大进展,但是必须将我们对其他基本细胞信号范式的理解进一步整合到组织工程方法中。已经证明生物电信号传导是跨器官系统的组织发育,再生和功能的重要组成部分,并且已知细胞外基质会改变细胞的生物电特性。因此,需要加强我们对基质和生物电信号如何相互作用以驱动细胞表型的理解。我们研究了如何通过同时改变静息膜电位和细胞外基质成分来改变心脏祖细胞的分化。儿科c-kit +心脏祖细胞在胎儿或成年心脏细胞外基质上分化,同时接受改变静息膜电位的药物治疗。平滑肌基因表达随着去极化而增加,而随着超极化而下降,而内皮和心脏的表达则保持不变。早期平滑肌蛋白表达会因基质发育年龄而改变,胎儿ECM似乎会放大静息膜电位的影响。因此,将基质组成和生物电信号结合起来,代表了在组织工程和再生医学中指导细胞行为的潜在选择。

更新日期:2020-08-05
down
wechat
bug