Squalene hopene cyclases catalyse the conversion of a linear substrate squalene to a cyclic product with high stereo-selectivity.The enzyme squalene hopene cyclase from Pseudomonas mendocina expressed in E. coli BL21 (DE3) was evaluated for its synthetic drug transforming ability. Nine synthetic drugs were selected as substrates for biotransformation reactions by the enzyme. The homology modelling of the protein and docking of the selected ligands were performed using GOLD suite docking software. The drug which showed maximum binding with the active-site residues of the enzyme was selected for biotransformation studies. On transformation with the enzyme, Glibenclamide, the selected antidiabetic drug alone showed significant changes in the FT/IR spectra; hence, it was selected for LCMS analysis to confirm the transformations. From the chromatogram and MS spectra, the mono-oxygenation of the product due to the enzymatic activity was confirmed. The drug transforming ability of the purified SHC could be used as an ideal tool for the generation of new and active substrate derivatives.

角鲨烯九烯环化酶催化线性底物角鲨烯向具有高立体选择性的环状产物的转化。门氏假单胞菌的角鲨烯九烯环化酶在大肠杆菌中表达评价了BL21（DE3）的合成药物转化能力。通过该酶选择了九种合成药物作为生物转化反应的底物。蛋白质的同源性建模和所选配体的对接使用GOLD套件对接软件进行。选择显示出与酶的活性位点残基最大结合的药物进行生物转化研究。用酶格列本脲转化后，仅所选的抗糖尿病药在FT / IR光谱上显示出明显变化；因此，选择它进行LCMS分析以确认转化。从色谱图和MS光谱中，确认了由于酶活性导致的产物的单加氧。