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Characterization of the glycans involved in sea urchin Paracentrotus lividus reversible adhesion
Marine Biology ( IF 2.4 ) Pub Date : 2020-08-05 , DOI: 10.1007/s00227-020-03707-9
Mariana Simão , Mariana Moço , Luís Marques , Romana Santos

Sea urchins have hundreds of specialized adhesive organs, the tube feet, which play a key role in locomotion, substrate attachment and food capture. Tube feet are composed by two functional units: a proximal cylindrical stem that is mobile and flexible, attached to a distal flattened disc that produces adhesive secretions. Oral tube feet discs possess a specialized duo-glandular epidermis that produces adhesive and de-adhesive secretions, enabling strong but reversible adhesion to the substrate. Due to the growing interest in biomimetic adhesives, several studies have been carried out to characterize sea urchin adhesives, and up to date, it has been shown that it is composed by proteins and glycans. The protein fraction has been the subject of several studies, that pin-pointed several adhesion-related candidates. Contrastingly, little is known about the glycans that compose sea urchin adhesives. This study aims at contributing to this topic by focusing on the characterization of the glycosidic fraction of the adhesive secreted by the sea urchin Paracentrotus lividus (Lamarck, 1816), using a battery of 22 lectins, applied to 3 complementary techniques. Our results show that five lectins label exclusively the disc adhesive epidermis and simultaneously the secreted adhesive, being, therefore, most likely relevant for sea urchin adhesion. In addition, it was possible to determine that the glycosidic fraction of the adhesive is composed by a high molecular weight glycoprotein containing N-acetylglucosamine oligomers.

中文翻译:

参与海胆 Paracentrotus lividus 可逆粘附的聚糖的表征

海胆有数百个特殊的粘附器官,即管足,它们在运动、基质附着和食物捕获中起着关键作用。管脚由两个功能单元组成:一个近端圆柱形茎,可移动和灵活,连接到远端扁平椎间盘,产生粘性分泌物。口腔管足盘具有特殊的双腺体表皮,可产生粘性和去粘性分泌物,从而能够牢固但可逆地粘附到基材上。由于对仿生粘合剂的兴趣日益浓厚,已经进行了多项研究来表征海胆粘合剂,迄今为止,已经表明它由蛋白质和聚糖组成。蛋白质部分是几项研究的主题,确定了几个与粘附相关的候选物。相比之下,人们对构成海胆粘合剂的聚糖知之甚少。本研究旨在通过重点表征海胆 Paracentrotus lividus (Lamarck, 1816) 分泌的粘合剂的糖苷部分,使用一组 22 种凝集素,应用于 3 种互补技术,从而为该主题做出贡献。我们的结果表明,五种凝集素专门标记椎间盘粘连表皮,同时标记分泌的粘连,因此,最有可能与海胆粘连有关。此外,可以确定粘合剂的糖苷部分由含有 N-乙酰氨基葡糖低聚物的高分子量糖蛋白组成。本研究旨在通过重点表征海胆 Paracentrotus lividus (Lamarck, 1816) 分泌的粘合剂的糖苷部分,使用一组 22 种凝集素,应用于 3 种互补技术,从而为该主题做出贡献。我们的结果表明,五种凝集素专门标记椎间盘粘连表皮,同时标记分泌的粘连,因此,最有可能与海胆粘连有关。此外,可以确定粘合剂的糖苷部分由含有 N-乙酰氨基葡糖低聚物的高分子量糖蛋白组成。本研究旨在通过重点表征海胆 Paracentrotus lividus (Lamarck, 1816) 分泌的粘合剂的糖苷部分,使用一组 22 种凝集素,应用于 3 种互补技术,从而为该主题做出贡献。我们的结果表明,五种凝集素专门标记椎间盘粘连表皮,同时标记分泌的粘连,因此,最有可能与海胆粘连有关。此外,可以确定粘合剂的糖苷部分由含有 N-乙酰氨基葡糖低聚物的高分子量糖蛋白组成。我们的结果表明,五种凝集素专门标记椎间盘粘连表皮,同时标记分泌的粘连,因此,最有可能与海胆粘连有关。此外,可以确定粘合剂的糖苷部分由含有 N-乙酰氨基葡糖低聚物的高分子量糖蛋白组成。我们的结果表明,五种凝集素专门标记椎间盘粘连表皮,同时标记分泌的粘连,因此,最有可能与海胆粘连有关。此外,可以确定粘合剂的糖苷部分由含有 N-乙酰氨基葡糖低聚物的高分子量糖蛋白组成。
更新日期:2020-08-05
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