Control of Brucella ovis infection in sheep flocks in the United States depends on early detection of B. ovis antibodies via serologic testing. We used 2,276 sheep sera and various cutoff values to compare seroprevalence and agreement between 2 ELISAs: the National Veterinary Services Laboratories (NVSL) B. ovis indirect ELISA and the IDEXX B. ovis ELISA kit. A subset of 295 sera was used to compare agreement and evaluate relative sensitivity and specificity of the 2 ELISAs with an agar gel immunodiffusion (AGID) test kit. There was no significant difference in B. ovis seroprevalence between the ELISAs; however, there was poor agreement between them. When the AGID test was used as the reference test, the IDEXX ELISA with a moderate cutoff value (S/P ratio = 45%) had the highest relative sensitivity of 38.1% and specificity of 92.0%. The NVSL ELISA with a lax cutoff value (S/P ratio = 0.75) had relative sensitivity of 19.1% and specificity of 94.6%. Receiver operating characteristic analysis revealed that optimal cutoff values for the NVSL and IDEXX ELISAs were 0.091 and 16.5%, respectively. This results in sensitivity and specificity of 85.7% and 31.8% for the NVSL ELISA, and sensitivity and specificity of 81.0% and 53.6% for the IDEXX ELISA, respectively.

在美国控制羊群中的布鲁氏菌感染取决于通过血清学检测及早检测到B. ovis抗体。我们使用 2,276 份绵羊血清和各种截断值来比较 2 种 ELISA 的血清阳性率和一致性：国家兽医服务实验室 (NVSL)羊双歧杆菌间接 ELISA 和 IDEXX羊双歧杆菌ELISA 试剂盒。使用琼脂凝胶免疫扩散 (AGID) 检测试剂盒，使用 295 份血清的子集来比较一致性并评估 2 种 ELISA 的相对灵敏度和特异性。B. ovis没有显着差异ELISA 之间的血清阳性率；然而，他们之间的共识很差。以AGID试验作为参考试验时，具有中等截断值（S/P比=45%）的IDEXX ELISA的相对灵敏度最高，为38.1%，特异性为92.0%。具有宽松截止值（S/P 比 = 0.75）的 NVSL ELISA 的相对灵敏度为 19.1%，特异性为 94.6%。接受者操作特征分析显示，NVSL 和 IDEXX ELISA 的最佳截止值分别为 0.091 和 16.5%。这导致 NVSL ELISA 的灵敏度和特异性分别为 85.7% 和 31.8%，以及 IDEXX ELISA 的灵敏度和特异性分别为 81.0% 和 53.6%。